Korean J Transplant.  2021 Oct;35(Supple 1):S68. 10.4285/ATW2021.PO-1142.

Ginsenoside Rg3 attenuates ischemia reperfusion induced renal injury in mice via induction of autophagy flux

• Shin JA
• Jeong Jy
• Hwang HB
• Han Sh
• Lee EJ
• Ham Y
• Na KR
• Lee KW
• Choi DE

• KMID: 2521408
• DOI: http://doi.org/10.4285/ATW2021.PO-1142

Abstract

Background
Ginsenoside Rg3 (Rg3) has been shown as protective effects via various mechanism. However, the reno-protective effect and the role of autophagy are not clearly evaluated. This study investigate Rg3 induces autophagy flux and reduces renal cell death in renal ischemia reperfusion injury (IRI).
Methods
C57Bl/6 mice were divided into the following groups: sham; Rg3 treated sham; saline treated IRI mice; Rg3 treated IRI mice. Kidneys and blood were collected 24 hours after operation of mice (sham and IR operation). Renal function, kidney histology, and the protein expression of autophagy signals were evaluated.
Results
In IRI mice, the levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were increased, compared to sham. The Rg3 treatment decreased the BUN and SCr in IRI mice. In addition, Rg3 treatment decreased the renal injury score including the renal tubular cell detachment and necrosis in IRI mice. Rg3 treated IRI mice showed significantly less oxidative stress and autophagy impairment, greater amounts of LC3 and Beclin-1, lower amounts of p62, and higher levels of renal ATP6E compared to saline treated IRI mice. Rg3 treatment also increased phosphorylation of AMPK in IRI mice kidney.
Conclusions
Rg3 has renoprotection against renal IR injury via enhancement of autophagy flux.