Korean J Transplant.  2023 Nov;37(Suppl 1):S232. 10.4285/ATW2023.F-8252.

Ginsenoside Rg3 attenuates ischemia reperfusion injury via adenosine monophosphate-activated protein kinase-mediated autophagy flux

• Lee KW
• Choi DE
• Han S
• Lee EJ
• Ham YR
• Park H
• Park K
• Park H
• Hwang Y
• Na KR

• KMID: 2551852
• DOI: http://doi.org/10.4285/ATW2023.F-8252

Abstract

Background
Ginsenoside Rg3 (Rg3) comes is extracted from the heat processing of protopanaxadiol ginsenosides, such as Rb1, Rb2, Rc and Rd from red ginseng. Rg3 has been reported that it attenuates various organ injury through adenosine monophosphate-activated protein kinase (AMPK)-mediate autophagy flux. We evaluate whether Rg3 regulate autophagy flux through the AMPK pathway in ischemia reperfusion (IR)-induced kidney injury.
Methods
C57Bl/6 mice were divided into the following groups: sham-operated, Rg3 sham, control IR mice, and Rg3 treated IR mice. Kidneys and blood were collected harvested 24 hours after operation of mice (sham and IR operation). Renal function, kidney histology, and the protein expression of autophagy signals were evaluated. We evaluated whether Rg3 lower renal damage in IR mice model through renal function, tissue histology and autophagy flux expression.
Results
The levels of blood urea nitrogen (BUN) and serum creatinine were increased in control IR mice, compared to sham mice. The Rg3 treatment decreased the BUN and serum creatinine in IR mice. In addition, Rg3 treatment decreased the renal injury score including the renal tubular cell detachment and necrosis in IR mice. treated IR mice kidney showed better renal cell survival, renal function, and pathological damage than those of IR mice kidneys. In addition, Rg3 treated IR mice kidney showed significantly less oxidative stress and autophagy impairment; greater amounts of microtubule-associated protein 1A/1B-light chain 3 (LC3)-II, Beclin-1; lower amounts of p62; and higher levels of renal Rab7 and ATP6E, compared to than IR mice kidney. Rg3 treatment. They also activates showed more AMPK activation, which resulted in the inhibition of phosphorylation of the mammalian target of rapamycin.
Conclusions
We report that Rg3 has renoprotection against renal IR injury via AMPK mediated autophagy flux.