Korean J Transplant.  2023 Nov;37(Suppl 1):S234. 10.4285/ATW2023.F-8273.

PGC1-alpha plays a role in hypothermic renal protection of renal fibrosis after acute kidney injury

• Park H
• Choi DE
• Park H
• Hwang Y
• Park K
• Lee EJ
• Ham YR
• Na RR
• Lee KW

• KMID: 2551854
• DOI: http://doi.org/10.4285/ATW2023.F-8273

Abstract

Background
PGC-1 is known to protect against renal injury. It has been known that hypothermia attenuates against the renal injury induced by ischemia reperfusion. Hypothermia induce PGC-1 in some organ injury models. We evaluated the role of PGC-1 in hypothermia protection against renal ischemia-reperfusion injury (IRI).
Methods
We prepared a fibrosis model by inducing IRI. C57BL/6 mice were divided into the following groups: sham mice and IRI mice (37C vs. 32C). The kidneys were harvested 20 minutes after the induction of renal ischemia and on day 1, day 3, day 7, and after IRI. Fibrosis markers and the renal injury score were evaluated.
Results
The blood urea nitrogen levels, and serum creatinine levels, and the histologic renal injury scores were significantly lower in the 32C IRI groups than in the 37C IRI groups. The protein levels of fibrosis markers were significantly decreased, while the BMP7 and PGC-1 level was significantly increased in the 32C IRI mice group. Hypothermia increased the PGC-1 both, in vivo and in vitro. Knock down of PGC-1 expression increased in vitro renal fibrosis.
Conclusions
Hypothermia attenuates renal fibrosis in renal IRI mice kidneys. PGC-1 may play a role in hypothermic protection in renal fibrosis following IRI.