Exp Mol Med.  2008 Dec;40(6):686-698. 10.3858/emm.2008.40.6.686.

Ginsenoside Rb1 attenuates intestinal ischemia-reperfusion- induced liver injury by inhibiting NF-kappa B activation

• Wang J
• Qiao L
• Li Y
• Yang G

• KMID: 1072949
• DOI: http://doi.org/10.3858/emm.2008.40.6.686

Abstract

Intestinal ischemia-reperfusion (I/R) is an important event in the pathogenesis of multiple organ dysfunction syndrome (MODS). The aim of this study is to determine the effects of ginsenoside Rb1 on liver injury induced by intestinal I/R in rats. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Liver and intestinal histology was observed. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) level in serum and malondialdehyde (MDA) level in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-alpha, MDA level and immunohistochemical expression of NF-kappa B and intracellular adhesion molecale-1 (ICAM-1) in liver tissues was assayed. In addition, a western blot analysis of liver NF-kappa B expression was performed. Results indicated intestinal I/R induced intestinal and liver injury, which was characterized by increase of AST and ALT in serum, MDA level in intestine, MPO, TNF-alpha and MDA level and ICAM-1 and NF-kappa B expression in the liver tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated liver injury, decreased MPO, TNF-alpha and MDA level, NF-kappa B and ICAM-1 expression in liver tissues. In conclusion, ginsenoside Rb1 ablated liver injury induced by intestinal I/R by inhibiting NF-kappaB activation.

Keyword

ginsenoside Rb1; intercellular adhesion molecule-1; NF-kappa B; reperfusion injury; tumor necrosis factor-alpha

MeSH Terms

Alanine Transaminase/blood
Animals
Aspartate Aminotransferases/blood
Biological Markers/metabolism
Ginsenosides/*pharmacology
Intercellular Adhesion Molecule-1/metabolism
Intestines/*blood supply/metabolism/pathology
Ischemia/metabolism/pathology
Liver/enzymology/pathology
Liver Diseases/etiology/*pathology
Male
Malondialdehyde/metabolism
NF-kappa B/*antagonists & inhibitors/metabolism
Peroxidase/metabolism
Rats
Rats, Wistar
Reperfusion Injury/complications/*pathology
Tumor Necrosis Factor-alpha/metabolism