J Vet Sci.
2021 Jul;22(4):e49. 10.4142/jvs.2021.22.e49.
Identification of a mimotope of an infectious bronchitis virus S1 protein
- Affiliations
-
- 1School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China
- KMID: 2518811
- DOI: http://doi.org/10.4142/jvs.2021.22.e49
Abstract
- The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzymelinked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
- Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand
- Genomic characteristics of natural recombinant infectious bronchitis viruses isolated in Korea
- Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea
- Construction of an avian hepatitis E virus replicon expressing heterologous genes and evaluation of its potential as an RNA vaccine platform
