J Vet Sci.  2009 Sep;10(3):219-223. 10.4142/jvs.2009.10.3.219.

Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

• Pohuang T
• Chansiripornchai N
• Tawatsin A
• Sasipreeyajan J

• KMID: 1726921
• DOI: http://doi.org/10.4142/jvs.2009.10.3.219

Abstract

Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and amino acid sequence identities less than 84% in common with IBVs published in the GenBank database. This group likely represents the strains indigenous to Thailand. The isolates in group II showed a close relationship with Chinese IBVs. They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV). This finding indicated that the recent Thai IBVs evolved separately and at least two groups of viruses are circulating in Thailand.

Keyword

infectious bronchitis virus; phylogenetic analysis; S1 gene; sequencing; Thailand

MeSH Terms

Animals
Chickens
Coronavirus Infections/*veterinary/virology
Infectious bronchitis virus/*genetics/*isolation & purification
Molecular Sequence Data
Phylogeny
Poultry Diseases/*virology
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Thailand
Viral Proteins/chemistry

Figure

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  Fig. 1 Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus isolates and published sequences.

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