J Vet Sci.  2021 Jul;22(4):e53. 10.4142/jvs.2021.22.e53.

Optimization of adipogenic differentiation conditions for canine adipose-derived stem cells

• Kim JY
• Park EJ
• Kim SM
• Lee HJ

• KMID: 2518799
• DOI: http://doi.org/10.4142/jvs.2021.22.e53

Abstract

Background
Canine adipose-derived stem cells (cADSCs) exhibit various differentiation properties and are isolated from the canine subcutaneous fat. Although cADSCs are valuable as tools for research on adipogenic differentiation, studies focusing on adipogenic differentiation methods and the underlying mechanisms are still lacking.
Objectives
In this study, we aimed to establish an optimal method for adipogenic differentiation conditions of cADSCs and evaluate the role of peroxisome proliferatoractivated receptor gamma (PPARγ) and estrogen receptor (ER) signaling in the adipogenic differentiation.
Methods
To induce adipogenic differentiation of cADSCs, 3 different adipogenic medium conditions, MDI, DRI, and MDRI, using 3-isobutyl-1-methylxanthine (M), dexamethasone (D), insulin (I), and rosiglitazone (R) were tested.
Results
MDRI, addition of PPARγ agonist rosiglitazone to MDI, was the most significantly facilitated cADSC into adipocyte. GW9662, an antagonist of PPARγ, significantly reduced adipogenic differentiation induced by rosiglitazone. Adipogenic differentiation was also stimulated when 17β-estradiol was added to MDI and DRI, and this stimulation was inhibited by the ER antagonist ICI182,780.
Conclusions
Taken together, our results suggest that PPARγ and ER signaling are related to the adipogenic differentiation of cADSCs. This study could provide basic information for future research on obesity or anti-obesity mechanisms in dogs.

Keyword

Dogs; canine adipose-derived stem cells; adipogenesis; PPAR gamma; estrogen receptor