Exp Mol Med.  2013 Jan;45(1):e6.

Regulation of osteogenic differentiation of human adipose-derived stem cells by controlling electromagnetic field conditions

  • 1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea. dwcho@postech.ac.kr
  • 2Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
  • 3Department of Mechanical Engineering, Korea Polytechnic University, Siheung, Korea. yhjeong@kpu.ac.kr
  • 4Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Korea.


Many studies have reported that an electromagnetic field can promote osteogenic differentiation of mesenchymal stem cells. However, experimental results have differed depending on the experimental and environmental conditions. Optimization of electromagnetic field conditions in a single, identified system can compensate for these differences. Here we demonstrated that specific electromagnetic field conditions (that is, frequency and magnetic flux density) significantly regulate osteogenic differentiation of adipose-derived stem cells (ASCs) in vitro. Before inducing osteogenic differentiation, we determined ASC stemness and confirmed that the electromagnetic field was uniform at the solenoid coil center. Then, we selected positive (30/45 Hz, 1 mT) and negative (7.5 Hz, 1 mT) osteogenic differentiation conditions by quantifying alkaline phosphate (ALP) mRNA expression. Osteogenic marker (for example, runt-related transcription factor 2) expression was higher in the 30/45 Hz condition and lower in the 7.5 Hz condition as compared with the nonstimulated group. Both positive and negative regulation of ALP activity and mineralized nodule formation supported these responses. Our data indicate that the effects of the electromagnetic fields on osteogenic differentiation differ depending on the electromagnetic field conditions. This study provides a framework for future work on controlling stem cell differentiation.


adipose-derived stem cells; electromagnetic field; frequency; magnetic flux density; optimization; osteogenic differentiation

MeSH Terms

Adipose Tissue/*cytology
Alkaline Phosphatase/metabolism
Biological Markers/metabolism
Bone Matrix/metabolism
Calcification, Physiologic/genetics
*Cell Differentiation/genetics
Core Binding Factor Alpha 1 Subunit/metabolism
*Electromagnetic Fields
Reproducibility of Results
Stem Cells/*cytology/enzymology/metabolism
Biological Markers
Core Binding Factor Alpha 1 Subunit
Alkaline Phosphatase
Full Text Links
  • EMM
export Copy
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr