J Vet Sci.
2020 Jul;21(4):e68. 10.4142/jvs.2020.21.e68.
A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
- Affiliations
-
- 1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
- 2College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China
- 3College of Animal Science and Technology, Shihezi University, Shihezi 832003, China
- 4University of Chinese Academy of Sciences, Beijing 100049, China
- KMID: 2504884
- DOI: http://doi.org/10.4142/jvs.2020.21.e68
Abstract
- A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus
- An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
- Intracellular Localization of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein
- Seroprevalence of Antibody to Procine Reproductive and Respiratory Syndrome Virus in Diagnostic Submissions
- Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus
