Precis Future Med.  2020 Mar;4(1):31-36. 10.23838/pfm.2019.00121.

The effects of three novel probiotics isolated from the Korean fermented food Kimchi on the stress-induced defecation of rats

• Kim MJ
• Min YW
• Lee C
• Jang YS
• Kim CH
• Chang DK

• KMID: 2501511
• DOI: http://doi.org/10.23838/pfm.2019.00121

Abstract

Purpose
The interest in novel probiotics that improve symptoms of irritable bowel syndrome (IBS) has been growing trend. This study aims to investigate the probiotic effect of three kinds of Lactobacillus strain that are isolated from kimchi.
Methods
We used Wister strain rats, male sex. The rat subjects were subdivided into four groups by which to be administered Lactobacillus casei DK128, Lactobacillus paracasei DK121, Lactobacillus platarum DK119 or vesicle only (control). Each Lactobacillus (1× 10⁹ colony forming unit) was administered by oral gavage once daily for 10 days. At day 10, the rats were stressed by being placed in confinement in a restraining stress cage for 1 hour. The stool of the animals were collected after the stress test. The stool weight was recorded and measured in grams, then, each data were transformed to a percentage as compared with the mean stool weight of same age in control group. The stool consistency was scored 0 to 4 (0, normal; 2, loose stool; 4, diarrhea). We compared the stool weight and consistency between the treatments.
Results
The total stool weight of the rats was significantly reduced in the L. casei DK128 group (65%, P = 0.0368) and the L. paracasei DK121 group (64%, P = 0.0138), as compared to the control group (100%). The stool consistency of the L. paracasei DK121 group was noted as being better than the control group (2 vs. 4, P= 0.0272).
Conclusion
Our findings suggest that L. casei DK128 and L. paracasei DK121 has the characteristics of probiotics that could control symptoms of IBS.

Keyword

Irritable bowel syndrome; Kimchi; Probiotics; Rats; Stress