J Korean Ophthalmol Soc.  2020 Mar;61(3):267-273. 10.3341/jkos.2020.61.3.267.

Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells

Affiliations
  • 1Department of Ophthalmology, Daegu Catholic University College of Medicine, Daegu, Korea. jwkim@cu.ac.kr

Abstract

PURPOSE
To investigate the effects of hydrogen sulfide (Hâ‚‚S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO).
METHODS
After exposing primary cultured HTMCs to 0, 50, 100, and 500 µM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 µM NaHS and 10 µM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of Hâ‚‚S and NO.
RESULTS
Greater than 100 µM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 µM NaHS and 10 µM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05).
CONCLUSIONS
NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, Hâ‚‚S at high concentrations may increase trabecular outflow, which may not be associated with NO.

Keyword

Hydrogen sulfide; Nitric oxide; Permeability; Trabecular meshwork

MeSH Terms

Humans
Hydrogen Sulfide*
Hydrogen*
Nitric Oxide*
Nitroprusside
Permeability*
RNA, Messenger
Sodium
Trabecular Meshwork*
Hydrogen
Hydrogen Sulfide
Nitric Oxide
Nitroprusside
RNA, Messenger
Sodium
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