Clin Exp Reprod Med.
2019 Dec;46(4):189-196. 10.5653/cerm.2019.00514.
Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system
- Affiliations
-
- 1CNC Biotech Inc., Suwon, Korea.
- 2Department of Biomedical Laboratory Science, Eulji University, Seongnam, Korea. junjh55@hanmail.net
- 3Department of Biomedical Laboratory Science, BK21 PLUS Program, Graduate School, Eulji University, Seongnam, Korea.
- 4Department of Senior Healthcare, BK21 PLUS Program, Graduate School, Eulji University, Seongnam, Korea.
- KMID: 2469595
- DOI: http://doi.org/10.5653/cerm.2019.00514
Abstract
OBJECTIVE
We aimed to evaluate the effects of different oxygen conditions (20% [high Oâ‚‚], 5% [low Oâ‚‚] and 5% decreased to 2% [dynamic Oâ‚‚]) on mouse pre- and peri-implantation development using a novel double-channel gas supply (DCGS) incubator (CNC Biotech Inc.) to alter the oxygen concentration during in vitro culture.
METHODS
The high-Oâ‚‚ and low-Oâ‚‚ groups were cultured from the one-cell to the blastocyst stage under 20% and 5% oxygen concentrations, respectively. In the dynamic-Oâ‚‚ group, mouse embryos were cultured from the one-cell to the morula stage under 5% Oâ‚‚ for 3 days, followed by culture under 2% Oâ‚‚ to the blastocyst stage. To evaluate peri-implantation development, the blastocysts from the three groups were individually transferred to a fibronectin-coated dish and cultured to the outgrowth stage in droplets.
RESULTS
The blastocyst formation rate was significantly higher in the low-Oâ‚‚ and dynamic-Oâ‚‚ groups than in the high-Oâ‚‚ group. The total cell number was significantly higher in the dynamic-Oâ‚‚ group than in the low-Oâ‚‚ and high-Oâ‚‚ groups. Additionally, the apoptotic index was significantly lower in the low-Oâ‚‚ and dynamic-Oâ‚‚ groups than in the high-Oâ‚‚ group. The trophoblast outgrowth rate and spread area were significantly higher in the low-Oâ‚‚ and dynamic-Oâ‚‚ groups than in the high-Oâ‚‚ group.
CONCLUSION
Our results showed that a dynamic oxygen concentration (decreasing from 5% to 2%) had beneficial effects on mouse pre- and peri-implantation development. Optimized, dynamic changing of oxygen concentrations using the novel DCGS incubator could improve the developmental competence of in vitro cultured embryos in a human in vitro fertilization and embryo transfer program.
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