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Comparative study on the development of frozen-thawed mouse embryo using vitrification at each developmental stage

Ahn KH, Oh JH, Lee JJ, Kim T, Kim SH

  • KMID: 2272438
  • Korean J Obstet Gynecol.
  • 2004 Nov;47(11):2159-2166.
OBJECTIVE: To find out the optimal freezing time in terms of developmental stage of mouse embryo and the effectiveness of vitrification method for freezing them. METHODS: Superovulation induction was performed using...
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Effects of Indomethacin on Development and Hatching of Mouse Embryo

Cheon YP, Gye MC, Kim CH, Kim MK

  • KMID: 1930554
  • Korean J Fertil Steril.
  • 1997 Apr;24(1):35-42.
The present study was designed to define the role of prostaglandin in the development and hatching of mouse embryo. The effects of indomethacin, an inhibitor of prostaglandin synthesis, on the...
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Vitrification of Mouse Embryos in Ethylene Glycol-based Solutions

Kim MY, Lee ES, Lee SW, Lee YI

  • KMID: 2085176
  • Korean J Fertil Steril.
  • 2005 Jun;32(2):177-185.
OBJECTIVE: This study was conducted to find an optimal condition for the vitrification of mouse morulae and expanded blastocysts. MATERIALS AND METHODS: Mouse embryos were obtained at 2-cell stage and cultured...
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Development of Mice Embryo after Cryopreservation and Thawing

Lee KS, Moon SY, Ahn JJ

  • KMID: 2075232
  • Korean J Obstet Gynecol.
  • 1997 Jul;40(7):1336-1347.
Cryopreservation has proved to be a highly successful method for long term storage of viable embryos and it is necessary for human embryo cryopreservation in assisted reproductive technology to reduce...
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Timed Analysis of Mouse 2 - Cell Embryo Development in Dulbecco's Modified Eagle Medium ( DMEM ) in The Presence of Glutamine With of Without Glucose

Chun SS, Park KS, Lee TH, Kim JH, Song HB

  • KMID: 2269972
  • Korean J Obstet Gynecol.
  • 2000 Mar;43(3):395-399.
OBJECTIVE: To evaluat the effects of a culture medium with glucose in the presence of glutamine on the development of mouse embryos. METHODS: Two-cell embryos recovered from ICR mice at 48...
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Telomeric Dynamics and Telomerase Activity in Early Bovine Embryos

Jung YH, Lee SH, Cho SR, Kong IK, Cho JD, Sohn SH

  • KMID: 2070990
  • Korean J Reprod Med.
  • 2009 Jun;36(2):101-109.
OBJECTIVE: This study was carried out to analyze the amount of telomeric DNA and telomerase activity in early bovine embryos. METHODS: The amount of telomeric DNA in early bovine embryos at...
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The Effect of EDTA and Fetal Cord Serum Supplementation to Ham's F-10 Culture Medium on Development Potential of Mouse Embryos in Vitro

Kim BS, Lee YG, Park YK, Lee TH, Lee SH

It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown...
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The Development of Human Embryos in the Buffalo Rat Liver(BRL) Cell Coculture

Lee TH

  • KMID: 2260776
  • Korean J Obstet Gynecol.
  • 1997 Apr;40(4):701-705.
Culture requirements for in vitro development of human preimplantation embryos have not been fully defined. Helper cells in coculture would pave the way for repro-ducible embryo culture system of in...
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The Effect of Nitric Oxide on the Embryonal Development in Mouse

Min BK, Kim KS, Rhee HS, Hong GY, Shin HD, Sung YK, Kim HM

  • KMID: 2304569
  • Korean J Fertil Steril.
  • 1998 Aug;25(2):109-113.
OBJECTIVE: To ananlyze the direct effect of nitric oxide (NO), generated from sodium prusside (SNP) on the embryo developments in reproductive process. DESIGN: ova from mouse were treated to allow fertilization...
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What is the Cause of Low End: Tidal CO2 Tension During General Endotracheal Anesthesia?

Lee SK, Kim JS

BACKGROUND: We have previously demonstrated the isoflurane and halothane may be detrimental to in vitro fertilization of mouse oocytes in high concentrations. The aim of this study is to compare...
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Comparative Effects of Halothane, Enflurane and Isoflurane on the Mouse Two cell Embryo Development

Kim JH, Lee CH

BACKGROUND: We have previously demonstrated the isoflurane and halothane may be detrimental to in vitro fertilization of mouse oocytes in high concentrations. The aim of this study is to compare...
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The Development of Early Mouse Embryos Depend on Ca2+ Concentration

Yang JS, Bae IH

  • KMID: 2085143
  • Korean J Fertil Steril.
  • 2000 Sep;27(3):275-282.
OBJECTIVE: This study was to determine the effect of different concentration of calcium in medium on the preimplantational development of zygotes and early 2-cell embryos. METHODS: Female mice of ICR strain...
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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice

Lee YS, Park MJ, Park SH, Koo JS, Moon HS, Joo BS

OBJECTIVE: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female...
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Differential Expression of Glucose Transporter Gene in Mouse Early Embryos

Youm HW, Byun HK, Song GJ, Kim HK, Lee HJ

  • KMID: 2085108
  • Korean J Fertil Steril.
  • 1998 Apr;25(1):77-86.
The uptake of glucose for metabolism and growth is essential to most animal cells and is mediated by glucose-transporter (GLUT) proteins. The aim of this study was to determine which...
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Expression of Apoptotic Genes in Mouse Preimplantation Embryo Development

Lee YI, Lee J, Kim MY, Chun SY

  • KMID: 2235703
  • Korean J Fertil Steril.
  • 2003 Mar;30(1):77-84.
OBJECTIVES: The aim of this study was to evaluate the influence of three different media on preimplatation embryo development and the expression of Bcl-2, Mcl-1, Bax, and Bok in mouse. MATERiALS...
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Influence of Epidermal Growth Factor on Expression of Matrix Metalloproteinase-2 , 9 mRNA in Mouse Embryos

Kim JH, Chae HD, Kang EH, Chu HS, Jeon YP, Kang BM, Chang YS

  • KMID: 2075703
  • Korean J Obstet Gynecol.
  • 1999 Apr;42(4):712-719.
OBJECTIVE: To investigate the influence of epidermal growth factor [EGF] on expression of matrix metalloproteinase-2, 9 [MMP-2, 9] mRNA in mouse embryos Materials and METHOD: From January to June 1998,...
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The effectiveness of ethylene glycol as cryoprotectant in mouse embryo freezing with slow freezing method

Kim YJ, Kim OK, Park EA, Hong SC, Yu SY, Kim T, Lee JJ, Oh JH, Kim SH

  • KMID: 2272847
  • Korean J Obstet Gynecol.
  • 2006 Jul;49(7):1481-1491.
OBJECTIVE: We intended to know how the cryoprotectant ethylene glycol (EG) would affect the outcome of the embryo development when used in slow freezing method. And to know if there...
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Study on Development of Mouse Preimplantation Embryos in Culture Media with Different Composition of Energy Sources

Lee JB, Kim JH, Ko JH, Oh YK, Son SK, Seo YS, Noh HT, Kang KC, Song HB, Lee KH

  • KMID: 2262662
  • Korean J Obstet Gynecol.
  • 2002 Mar;45(3):416-423.
OBJECTIVE: The objective of this study was to examine the effect on development of mouse preimplantation embryos in culture media with different composition of energy sources in vitro culture. METHODS: Two...
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In vitro Fertilization and Embryo Development in Simple Media of the Frozen-Thawed Cumulus-free Mouse Oocytes Cryopreserved by Vitrification

Jung SK, Kim SK, Lee JJ, Oh JH, Lee YH, Kim SH

  • KMID: 1566257
  • Korean J Fertil Steril.
  • 2002 Sep;29(3):201-208.
OBJECTIVE: To observe the capability of fertilization and embryo development including blastocyst formation of the oocytes in simple media after thawing of the cryopreserved cumulus-free mouse oocytes by vitrification method. METHODS:...
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Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Lee SC, Seo HC, Lee J, Jun JH, Choi KW

OBJECTIVE: We aimed to evaluate the effects of different oxygen conditions (20% [high Oâ‚‚], 5% [low Oâ‚‚] and 5% decreased to 2% [dynamic Oâ‚‚]) on mouse pre- and peri-implantation development...
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