Clin Exp Reprod Med.  2015 Jun;42(2):51-57. 10.5653/cerm.2015.42.2.51.

Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice

• Lee YS
• Park MJ
• Park SH
• Koo JS
• Moon HS
• Joo BS

• KMID: 1879364
• DOI: http://doi.org/10.5653/cerm.2015.42.2.51

Abstract

OBJECTIVE
This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.
METHODS
Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 microm.
RESULTS
In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.
CONCLUSION
These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

Keyword

Blastocyst formation; Hatching; Laser-assisted zona thinning; Multipoint laser-assisted zona thinning

MeSH Terms

Animals
Blastocyst
Embryonic Structures*
Female
Herpes Zoster*
Humans
Mice*
Morula
Zona Pellucida