Clin Exp Reprod Med.  2017 Dec;44(4):193-200. 10.5653/cerm.2017.44.4.193.

Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

Affiliations
  • 1Department of Obstetrics and Gynecology, Chungnam National University Hospital, Daejeon, Korea. good79ok@naver.com
  • 2Mirae i Infertility Clinic, Mirae Ladies Hospital, Daejeon, Korea.

Abstract


OBJECTIVE
This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers.
METHODS
First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed.
RESULTS
The hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p < 0.05). In the control-8 group (22.1±4.6), the cell number of the inner cell mass was higher than in the LAH groups (p < 0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group (92.8±8.9) than in the others (p < 0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group (19.5±5.1, p < 0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group (73.2±12.1) than in the other groups, but without statistical significance.
CONCLUSION
When LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.

Keyword

Blastulation; Laser assisted hatching; Mouse embryo; Vitrification

MeSH Terms

Animals
Blastocyst
Blastomeres
Cell Count
Embryonic Development*
Embryonic Structures*
Female
Flushing
Herpes Zoster
Mice*
Oviducts
Pregnancy
Vitrification*
Full Text Links
  • CERM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr