J Bacteriol Virol.  2019 Sep;49(3):124-132. 10.4167/jbv.2019.49.3.124.

Recharacterization of the Canine Adenovirus Type 1 Vaccine Strain based on the Biological and Molecular Properties

Affiliations
  • 1Viral Disease Research Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon, 39660, Republic of Korea. yangdk@korea.kr
  • 2KBNP Technology Institute, KBNP, Yesan-gun, 32417, Republic of Korea.

Abstract

Canine adenovirus type 1 (CAV-1) infection results in hepatitis in dogs. In this study, we investigated the biologic and genetic characteristics of the CAV-1 vaccine strain (CAV1V) to improve quality control about CAV vaccine. The identity of CAV1V as CAV-1 was confirmed based on its cytopathic effects and the results of hemagglutination (HA) and immunofluorescence assays, and electron microscopy. The CAV1V strain reached 10(7.5) TCID(50)/mL in MDCK cells at 4 days post-inoculation and exhibited hemmagglutination activity of 256 U using guinea pig erythrocytes. Intranuclear fluorescence in the infected cells was observed and typical adenoviruses were observed in electon microscope. CAV1V strain was identified as a CAV-1 strain by nucleotide sequence analysis. In a comparison of the nucleotide sequences of the fiber genes of several CAV strains, CAV1V showed the highest similarity (99.8%) with the GLAXO strain, which was isolated in Canada. Our biological characterization of CAV1V will facilitate quality control of the canine hepatitis vaccine.

Keyword

Canine adenovirus type 1; Genetic characterization; Vaccine

MeSH Terms

Adenoviridae
Adenoviruses, Canine*
Animals
Base Sequence
Canada
Dogs
Erythrocytes
Fluorescence
Fluorescent Antibody Technique
Guinea Pigs
Hemagglutination
Hepatitis
Madin Darby Canine Kidney Cells
Microscopy, Electron
Quality Control
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