Tissue Eng Regen Med.  2019 Dec;16(6):653-665. 10.1007/s13770-019-00212-z.

Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

• Liu W
• Zhang SN
• Hu ZQ
• Feng SM
• Li ZH
• Xiao SF
• Wang HS
• Li L

• KMID: 2467057
• DOI: http://doi.org/10.1007/s13770-019-00212-z

Abstract

BACKGROUND
With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects.
METHODS
After the decellularization of human arterial blood vessels, the cells were cultured with GFP⁺ (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin-eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot.
RESULTS
After 4 weeks (w) of co-culture of HAAM and GFP? porcine bile duct epithelial cells, GFP⁺ porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed.
CONCLUSION
The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.

Keyword

Tissue engineering; Human acellular arterial matrix; Common bile duct defect

MeSH Terms

Bile
Bile Ducts
Blood Vessels
Blotting, Western
Cholangiopancreatography, Magnetic Resonance
Cholecystectomy, Laparoscopic
Coculture Techniques
Common Bile Duct
Duodenum
Epithelial Cells
Fluorescent Antibody Technique
Humans*
Infant, Newborn
Liver
Methods
Microscopy, Electron
Polymerase Chain Reaction
Swine
Tissue Engineering