Int J Stem Cells.  2019 Nov;12(3):484-496. 10.15283/ijsc19090.

Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions

Affiliations
  • 1Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Korea. ks66kim@hanyang.ac.kr
  • 2Soonchunhyang Institute of Medi-bioscience, Soonchunhyang University, Cheonan, Korea.
  • 31st Research Center, Axceso Biopharma Co., Ltd., Yongin, Korea.
  • 4Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Korea. shhong@kangwon.ac.kr
  • 5College of Medicine, Hanyang University, Seoul, Korea.

Abstract

Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have established a variety of culture systems in a defined combination, xeno-free matrix and medium that supports the growth and differentiation of hPSCs. Here we described detailed hPSCs culture methods under feeder-free and chemically defined conditions using vitronetin and TeSR-E8 medium including supplement bioactive lysophospholipid for promoting hPSCs proliferation and maintaining stemness.

Keyword

Embryonic stem cells; Induced pluripotent stem cells; Feeder-free; Chemically defined conditions; Extracellular matrices

MeSH Terms

Animals
Cell Culture Techniques
Embryonic Stem Cells
Extracellular Matrix
Feeder Cells
Human Embryonic Stem Cells
Humans
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Stem Cells
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