Int J Stem Cells.  2019 Nov;12(3):463-473. 10.15283/ijsc19007.

Role of a 19S Proteasome Subunit- PSMD10(Gankyrin) in Neurogenesis of Human Neural Progenitor Cells

Affiliations
  • 1Advance Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, India. vprasanna@actrec.gov.in
  • 2Faculty of Biology, Technion – Israel Institute of Technology, Haifa, Israel.
  • 3Homi Bhabha National Institute, BARC Training School Complex, Mumbai, Maharashtra, India.
  • 4Department of Pathology, University of Massachusetts Medical School, Worcester, MA, USA.
  • 5Department of Physics, Bose Institute, Kolkata, India. soumen@jcbose.ac.in

Abstract

PSMD10(Gankyrin), a proteasome assembly chaperone, is a widely known oncoprotein which aspects many hall mark properties of cancer. However, except proteasome assembly chaperon function its role in normal cell function remains unknown. To address this issue, we induced PSMD10(Gankyrin) overexpression in HEK293 cells and the resultant large-scale changes in gene expression profile were analyzed. We constituted networks from microarray data of these differentially expressed genes and carried out extensive topological analyses. The overrecurring yet consistent theme that appeared throughout analysis using varied network metrics is that all genes and interactions identified as important would be involved in neurogenesis and neuronal development. Intrigued we tested the possibility that PSMD10(Gankyrin) may be strongly associated with cell fate decisions that commit neural stem cells to differentiate into neurons. Overexpression of PSMD10(Gankyrin) in human neural progenitor cells facilitated neuronal differentiation via β-catenin Ngn1 pathway. Here for the first time we provide preliminary and yet compelling experimental evidence for the involvement of a potential oncoprotein - PSMD10(Gankyrin), in neuronal differentiation.

Keyword

Gankyrin; Human neural progenitor cells; Microarray; Neurogenesis; Proteasome; PSMD10

MeSH Terms

HEK293 Cells
Humans*
Neural Stem Cells
Neurogenesis*
Neurons
Proteasome Endopeptidase Complex*
Stem Cells*
Transcriptome
Proteasome Endopeptidase Complex
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