Ann Lab Med.  2020 Mar;40(2):142-147. 10.3343/alm.2020.40.2.142.

Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar

Affiliations
  • 1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea. Hyelee@yonsei.ac.kr
  • 2International Tuberculosis Research Center, Changwon, Korea.
  • 3Department of Laboratory Medicine, Pusan National University Yangsan Hospital, Yangsan, Korea.

Abstract

BACKGROUND
Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. we developed QuantaMatrix Multiplexed Assay Platform (QMAP) MDR/XDR assay (QuantaMatrix Inc., Seoul, Korea) that can simultaneously detect mutations related to both first- and second-line drug resistance (rifampin, isoniazid, ethambutol, fluoroquinolones, second-line injectable drugs, and streptomycin).
METHODS
We used 190 clinical Mycobacterium tuberculosis (MTB) strains isolated from Myanmar, compared QMAP and pDST results, and determined concordance rates. Additionally, we performed sequence analyses for discordant results.
RESULTS
QMAP results were 87.9% (167/190) concordant with pDST results. In the 23 isolates with discordant results, the QMAP and DNA sequencing results completely matched.
CONCLUSIONS
The QMAP MDR/XDR assay can detect all known DNA mutations associated with drug resistance for both MDR- and XDR-MTB strains. It can be used for molecular diagnosis of MDR- and XDR-TB to rapidly initiate appropriate anti-TB drug therapy.

Keyword

Mycobacterium tuberculosis; Multidrug-resistant tuberculosis; Extensively drug-resistant tuberculosis; QuantaMatrix Multiplexed Assay Platform

MeSH Terms

Diagnosis*
DNA
Drug Resistance
Drug Therapy
Ethambutol
Extensively Drug-Resistant Tuberculosis*
Fluoroquinolones
Incidence
Isoniazid
Myanmar*
Mycobacterium tuberculosis
Phenotype
Seoul
Sequence Analysis
Sequence Analysis, DNA
Tuberculosis
Tuberculosis, Multidrug-Resistant
DNA
Ethambutol
Fluoroquinolones
Isoniazid

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