A Study on Serum Leptin Values by Elisa Method in Children

Song, Soo Ho; Chung, Young Hun

Abstract

PURPOSE
Leptin is an adipocyte-derived blood-borne satiety factor that acts on its cognate leptin receptor in the hypothalamus, thereby regulating food intake and energy expenditure. We measured the leptin concentrations in serum of normal and obese children with human leptin ELISA kit, unlike previous study with leptin RIA kit and investigated the relationship between leptin concentrations and body mass index, gender, and age.
METHODS
We measured serum concentrations of leptin in 67 children who were visited to the Department of Pediatrics, Chungnam National University Hospital during the period of 5 months from February, 1999 to June, 1999. Height, weight, obesity index, and body mass index were measured in 67 subjects. Leptin values in serum were measured by sandwich ELISA method. Data analysis was done according to the obesity, body mass index, gender and age.
RESULTS
The mean concentration of leptin was 7.69±8.83 ng/ml in normal children group and 36.34±18.57 ng/ml in obese group. Serum leptin concentrations were significant correlation with the body mass index (p < 0.01). Serum leptin concentration was significant higher in the group of over 10 years of age (p < 0.01). Leptin levels showed no significant difference by gender.
CONCLUSION
Serum leptin levels were significantly higher in obesity group than in control one, and they were correlated with body mass index and age. Measurements of leptin value by sandwich ELISA method are very useful and easily applicable to determine obesity.