Lab Anim Res.  2018 Dec;34(4):270-278. 10.5625/lar.2018.34.4.270.

Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

Affiliations
  • 1College of Veterinary Medicine, Kyungpook National University, Daegu, Korea. kskim728@knu.ac.kr
  • 2Department of Health and Exercise Science, Korea National Sport University, Seoul, Korea.
  • 3Biomedical Science Institute, Changwon National University, Changwon, Korea.
  • 4Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea.
  • 5College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
  • 6Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Korea.

Abstract

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCOâ‚‚), mean arterial blood pressure, heart rate, oxygen saturation (SpOâ‚‚), body temperature, pH, PCOâ‚‚, and POâ‚‚ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, SPOâ‚‚, arterial blood PCOâ‚‚, and POâ‚‚, which decreased after TBE anesthesia, or in heart rate and ETCOâ‚‚, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.

Keyword

Anesthesia effect; Korl:ICR mice; pulse oximeter; sex difference; tribromoethanol

MeSH Terms

Anesthesia
Anesthetics*
Animals
Arterial Pressure
Biomarkers
Body Temperature
Female
Heart Rate
Humans
Hydrogen-Ion Concentration
Kidney
Liver
Lung
Male
Mice
Mice, Inbred ICR*
Oxygen
Sex Characteristics
Anesthetics
Biomarkers
Oxygen
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