Lab Anim Res.  2011 Sep;27(3):197-203. 10.5625/lar.2011.27.3.197.

Kinetics of proinflammatory cytokines after intraperitoneal injection of tribromoethanol and a tribromoethanol/xylazine combination in ICR mice

• Cho YJ
• Lee YA
• Lee JW
• Kim JI
• Han JS

• KMID: 1850046
• DOI: http://doi.org/10.5625/lar.2011.27.3.197

Abstract

Tribromoethanol (2,2,2-tribromoethanol, TBE) is a popular injectable anesthetic agent used in mice in Korea. Our goal was to assess the risks associated with side effects (lesions) in the abdominal cavity, especially at high doses. To understand the underlying pathophysiological changes, we examined levels of cytokines through ELISA of abdominal lavage fluid and spleen collected from mice treated with low and high-dose TBE. ICR mice were anesthetized using one of the following protocols: a combination of TBE 200 mg/kg (1.25%) and xylazine 10 mg/kg; TBE 400 mg/kg (1.25%); and TBE 400 mg/kg (2.5%). Administration of high-dose TBE (400 mg/kg) increased the interleukin-1beta and interleukin-6 levels in the peritoneal cavity over the short term (<1 day) compared with sham controls and low-dose TBE (200 mg/kg) groups. Cytokine expression in the low-dose TBE group was similar to the control group, whereas in the high-dose TBE group cytokine levels were higher in abdominal lavage fluid and spleen over the long term (10 days post-injection). We conclude that a combination of TBE 200 mg/kg (1.25%) and xylazine (10 mg/kg) is a safe and effective anesthetic for use in animals.

Keyword

Anesthetic; cytokine; tribromoethanol; xylazine

MeSH Terms

Abdominal Cavity
Animals
Cytokines
Enzyme-Linked Immunosorbent Assay
Ethanol
Injections, Intraperitoneal
Interleukin-1beta
Interleukin-6
Kinetics
Korea
Mice
Mice, Inbred ICR
Peritoneal Cavity
Salicylamides
Spleen
Therapeutic Irrigation
Xylazine
Cytokines
Ethanol
Interleukin-1beta
Interleukin-6
Salicylamides
Xylazine

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