Gut Liver.  2019 Jul;13(4):430-439. 10.5009/gnl18316.

Bifidobacterium infantis Induces Protective Colonic PD-L1 and Foxp3 Regulatory T Cells in an Acute Murine Experimental Model of Inflammatory Bowel Disease

• Zhou L
• Liu D
• Xie Y
• Yao X
• Li Y

• KMID: 2458527
• DOI: http://doi.org/10.5009/gnl18316

Abstract

BACKGROUND/AIMS
The current study aims to investigate the protective effects of Bifidobacterium infantis on the abnormal immune response to inflammatory bowel disease (IBD) in dextran sodium sulfate (DSS)-induced colitis.
METHODS
Eight-week-old BALB/c mice were separated into five groups at random (control, DSS, DSS+B9 [B. infantis 1×10⁹ CFU], DSS+B8 [B. infantis 1×10⁸ CFU], and DSS+B7 [B. infantis 1×10⁷ CFU]). Colitis was induced by 5% DSS ad libitum for 7 days, at which time we assessed weight, the disease activity index (DAI) score, and the histological damage score. The nuclear transcription factor Foxp3 (a marker of Treg cells), cytokines interleukin-10 (IL-10) and transforming growth factor β1 (TGF-β1), and related proteins (programmed cell death ligand 1 [PD-L1] and programmed cell death 1 [PD-1]) were detected by an immunohistochemical method and Western blot.
RESULTS
B. infantis increased weight, decreased DAI scores and histological damage scores, increased the protein expression of Foxp3 (p<0.05) and cytokines IL-10 and TGF-β1 in mouse colon tissue (p<0.05), and increased the expression of PD-L1 in the treatment groups relative to that in the DSS group (p<0.05). The effect of B. infantis on Foxp3 and PD-L1 was dose dependent in the treatment groups (p<0.05). PD-L1 was positively correlated with Foxp3, IL-10, and TGF-β1.
CONCLUSIONS
In a mouse model of IBD, B. infantis can alleviate intestinal epithelial injury and maintain intestinal immune tolerance and thus may have potential therapeutic value for the treatment of immune damage in IBD.

Keyword

Bifidobacterium infantis; Inflammatory bowel disease; Programmed cell death ligand 1; T-Lymphocytes, regulatory

MeSH Terms

Animals
Bifidobacterium*
Blotting, Western
Cell Death
Colitis
Colon*
Cytokines
Dextrans
Immune Tolerance
Inflammatory Bowel Diseases*
Interleukin-10
Methods
Mice
Models, Theoretical*
Sodium
T-Lymphocytes, Regulatory*
Transcription Factors
Transforming Growth Factors
Cytokines
Dextrans
Interleukin-10
Sodium
Transcription Factors
Transforming Growth Factors