Immune Netw.  2010 Dec;10(6):247-249. 10.4110/in.2010.10.6.247.

Presence of Foxp3-expressing CD19(+)CD5(+) B Cells in Human Peripheral Blood Mononuclear Cells: Human CD19(+)CD5(+)Foxp3(+) Regulatory B Cell (Breg)

• Noh J
• Choi WS
• Noh G
• Lee JH

• KMID: 2150681
• DOI: http://doi.org/10.4110/in.2010.10.6.247

Abstract

Foxp3 is a transcript factor for regulatory T cell development. Interestingly, Foxp3-expressing cells were identified in B cells, especially in CD19(+)CD5(+) B cells, while those were not examined in CD19(+)CD5(-) B cells. Foxp3-expressing CD5(+) B cells in this study were identified in human PBMCs and were found to consist of 8.5+/-3.5% of CD19(+)CD5(+) B cells. CD19(+)CD5(+)Foxp3(+) B cells showed spontaneous apoptosis. Rare CD19(+)CD5(+) Foxp3(+) regulatory B cell (Breg) population was unveiled in human peripheral blood mononuclear cells and suggested as possible regulatory B cells (Breg) as regulatory T cells (Treg). The immunologic and the clinical relevant of Breg needs to be further investigated.

Keyword

Foxp3; Regulatory B cell; Breg; CD5; CD19; CD5(+) B

MeSH Terms

Apoptosis
B-Lymphocytes
B-Lymphocytes, Regulatory
Humans
T-Lymphocytes, Regulatory

Figure

• 

  Figure 1 Foxp3 expression in only CD5(+) cells in B cells. B cells were subgated by the surface CD19 expression and analyzed by the expression of CD5 and Foxp3.

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  Figure 2 Characteristics of Foxp3-expressing cells in CD19(+) B cells. (A) Foxp3(+) cell % in CD19(+)CD5(+) and CD19(+)CD5(-) B cell subpopulations. (B) Apoptotic characteristics of CD19(+)CD5(+) Foxp3(+) regulatory B cells (Breg) in a representative case. PBMCs were gated by the expression of CD19 and CD5. Each set of CD19 (+)CD5(-) B cells and CD19(+)CD5(+) B cells, CD19(-)CD5(-) cell was analyzed for Foxp3 expression and annexin V staining.

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