Change of Peripheral Blood CD5+ B Lymphocytes in Early Neonatal Period

Lee, Ye Ho; Cho, Bin; Lee, Won Bae; Whang, Kyung Tai

Abstract

PURPOSE: This study was aimed to investigate age-related changes of CD5+ B lymphocytes in healthy Korean neonates.
METHODS
Sixty healthy neonatal infants were enrolled in this study; at birth(n=10), day 1(n=10), day 2(n=10), day 3(n=10), day 4 (n=10) and day 5 (n=10). Phenotypic analysis of CD5+ B lymphocytes was performed on blood samples using standard flow-cytometric techniques.
RESULTS
The percentage of lymphocyte was significantly increased from at birth to day 5. The percentage of CD19+ lymphocyte was decreased from at birth to day 4, but statistically not significant. The percentage of CD5+ /CD19- lymphocyte on day 5 was significantly lower than that of at birth. The percentage of CD5-/CD19+ lymphocyte was not changed. The percentage of CD5+ /CD19- lymphocyte on day 1 was significantly higher than that of at birth, then it gradually decreased with aging to the at birth figure. The percentage of CD5+ /CD19+ lymphocyte was decreased from at birth to day 5, but the changes were not significant. The percentage of CD5+ / CD19+ lymphocyte in CD19+ lymphocyte was decreased from at birth to day 5, but it was not statistically significant.
CONCLUSION
CD5+ B lymphocyte counts are considerably higher than previously established adult values and the age-related changes are very important in interpretating CD5+ B lymphocyte. These data may serve as a reference range for studies in early neonatal period.