J Menopausal Med.  2018 Dec;24(3):169-175. 10.6118/jmm.2018.24.3.169.

Flavonoids Fraction of Mespilus Germanica Alleviates Insulin Resistance in Metabolic Syndrome Model of Ovariectomized Rats via Reduction in Tumor Necrosis Factor-α

• Kouhestani S
• Zare S
• Babaei P

• KMID: 2455890
• DOI: http://doi.org/10.6118/jmm.2018.24.3.169

Abstract

OBJECTIVES
The rate of metabolic syndrome (MetS) in women diagnosed as they age is one of the main concerns of health cares. Recently new strategies used to prevent progressions of MetS toward the diagnosis of diabetes have focused on plant flavonoids. This study was aimed to investigate the beneficial effects of flavonoids fraction of Mespilus germanica leaves (MGL) on MetS in ovariectomized (OVX) rats.
METHODS
Twenty-four adult female Wistar rats, weighing 200 to 250 g, were divided into 3 groups: Sham surgery, OVX + Salin, or OVX + Flavonoid. Three weeks after ovariectomy, animals displayed MetS criteria received flavonoid injection (10 mg/kg, intraperitoneally) for 21 days. Then the body weight, body mass index, waist circumference, visceral fat, fasting blood glucose, serum insulin, lipid profiles and tumor necrosis factor-α (TNF-α) were measured.
RESULTS
Treatment with flavonoids fraction of MGL significantly decreased serum level of insulin (P = 0.011), glucose (P = 0.024), TNF-α (P = 0.010), also MetS Z score (P = 0.020) and homeostasis model assessment of insulin resistance (P = 0.007). Lipid profiles and visceral fat showed insignificant reduction.
CONCLUSIONS
Flavonoids of MGL attenuates some of the MetS components possibly via reduction in TNF-α inflammatory cytokine.

Keyword

Menopause; Metabolic syndrome; Ovariectomy; Polyphenols

MeSH Terms

Adult
Animals
Blood Glucose
Body Mass Index
Body Weight
Diagnosis
Fasting
Female
Flavonoids*
Glucose
Homeostasis
Humans
Insulin Resistance*
Insulin*
Intra-Abdominal Fat
Menopause
Necrosis*
Ovariectomy
Plants
Polyphenols
Rats*
Rats, Wistar
Waist Circumference
Blood Glucose
Flavonoids
Glucose
Insulin
Polyphenols

Figure

• 

  Fig. 1 The effect of treatment with flavonoid of Mespilus germanica leaves (10 mg/kg) on triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), and low density lipoprotein (LDL). The data is presented as the mean ± standard error for 8 animals per group. TG *P = 0.033, TC *P = 0.047, HDL *P = 0.018, and non-LDL *P = 0.047, ovariectomized (OVX) + saline (SAL) group compared with sham-operated (SHAM) group. FLA: flavonoid.

• 

  Fig. 2 The effect of treatment with flavonoid of Mespilus germanica leaves (10 mg/kg) on: (A) fasting glucose *P = 0.011, ovariectomized (OVX) + saline (SAL) group compared with sham-operated (SHAM) group, †P = 0.024, OVX + flavonoid (FLA) group compared with OVX+SAL group, (B) fasting insulin *P = 0.048, OVX+SAL group compared with SHAM group, †P = 0.011, OVX+FLA group compared with OVX+SAL group, (C) homeostasis model assessment estimate of insulin resistance (HOMA-IR) *P = 0.004, OVX+SAL group compared with SHAM group, †P = 0.007, OVX+FLA group compared with OVX+SAL group. Values are reported as the mean ± standard error for 8 animals per group.

• 

  Fig. 3 The effect of treatment with flavonoid of Mespilus germanica leaves (10 mg/kg) on metabolic syndrome (MetS) Z score. The data is presented as the mean ± standard error for 8 animals per group. *P = 0.003, ovariectomized (OVX) + saline (SAL) group compared with sham-operated (SHAM) group, †P = 0.020, OVX+flavonoid (FLA) group compared with OVX + SAL group.

• 

  Fig. 4 The effect of treatment with flavonoid of Mespilus germanica leaves (10 mg/kg) on serum tumor necrosis factor-α (TNF-α). The data is presented as the mean ± standard error for 8 animals per group. *P = 0.035, ovariectomized (OVX) + saline (SAL) group compared with sham-operated (SHAM) group, †P = 0.010 OVX + flavonoid (FLA) group compared with OVX + SAL group.

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