Mycobiology.
2019 Jun;47(2):207-216. 10.1080/12298093.2019.1620975.
AcuD Gene Knockout Attenuates the Virulence of Talaromyces marneffei in a Zebrafish Model
- Affiliations
-
- 1Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. xiliyan@mail.sysu.edu.cn
- 2Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA.
- 3Division of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, RI, USA.
- 4Dermatology Hospital of Southern Medical University, Guangzhou, China.
- KMID: 2455701
- DOI: http://doi.org/10.1080/12298093.2019.1620975
Abstract
- Talaromyces marneffei is the only dimorphic species in its genus and causes a fatal systemic mycosis named talaromycosis. Our previous study indicated that knockdown of AcuD gene (encodes isocitrate lyase of glyoxylate bypass) of T. marneffei by RNA interference approach attenuated the virulence of T. marneffei, while the virulence of the AcuD knockout strains was not studied. In this study, T. marneffei-zebrafish infection model was successfully established through hindbrain microinjection with different amounts of T. marneffei yeast cells. After co-incubated at 28°C, the increasing T. marneffei inoculum doses result in greater larval mortality; and hyphae generation might be one virulence factor involved in T. marneffei-zebrafish infection. Moreover, the results demonstrated that the virulence of the ΔAcuD was significantly attenuated in this Zebrafish infection model.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Zebrafish and Mycobacterial infection
- Penicillium Species Other Than Talaromyces marneffei Producing Red Pigment from Clinical Specimens: Isolation of Talaromyces albobiverticillius
- Possibility of Zebrafish as New infection model for Leprosy
- Chronic Reserpine Administration for Depression Modeling in Zebrafish
- Zebrafish as an Emerging Model for Dyslipidemia and Associated Diseases
