Mycobiology.  2019 Jun;47(2):207-216. 10.1080/12298093.2019.1620975.

AcuD Gene Knockout Attenuates the Virulence of Talaromyces marneffei in a Zebrafish Model

Affiliations
  • 1Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. xiliyan@mail.sysu.edu.cn
  • 2Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA.
  • 3Division of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, RI, USA.
  • 4Dermatology Hospital of Southern Medical University, Guangzhou, China.

Abstract

Talaromyces marneffei is the only dimorphic species in its genus and causes a fatal systemic mycosis named talaromycosis. Our previous study indicated that knockdown of AcuD gene (encodes isocitrate lyase of glyoxylate bypass) of T. marneffei by RNA interference approach attenuated the virulence of T. marneffei, while the virulence of the AcuD knockout strains was not studied. In this study, T. marneffei-zebrafish infection model was successfully established through hindbrain microinjection with different amounts of T. marneffei yeast cells. After co-incubated at 28°C, the increasing T. marneffei inoculum doses result in greater larval mortality; and hyphae generation might be one virulence factor involved in T. marneffei-zebrafish infection. Moreover, the results demonstrated that the virulence of the ΔAcuD was significantly attenuated in this Zebrafish infection model.

Keyword

Talaromycosis; AcuD; virulence; zebrafish

MeSH Terms

Gene Knockout Techniques*
Hyphae
Isocitrate Lyase
Microinjections
Mortality
Rhombencephalon
RNA Interference
Talaromyces*
Virulence*
Yeasts
Zebrafish*
Isocitrate Lyase
Full Text Links
  • MB
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr