Nat Prod Sci.  2019 Jun;25(2):130-135. 10.20307/nps.2019.25.2.130.

Chemical Constituents of Impatiens balsamina Stems and Their Biological Activities

• Kim DH
• Lee TH
• Subedi L
• Kim SY
• Lee KR

• KMID: 2452782
• DOI: http://doi.org/10.20307/nps.2019.25.2.130

Abstract

The purification of the MeOH extract from Impatiens balsamina by repeated column chromatography led to the isolation of one new tetrahydronaphthalene (1), together with eleven known compounds (2 - 12). The structure of the new compound (1) was determined by spectral data analysis (1H and 13C-NMR, 1H-1H COSY, HSQC, HMBC, NOESY, and HR-ESI-MS). Isolated compounds (1 - 12) were evaluated for their inhibitory effects on NO production in LPS-activated murine microglial BV-2 cells and their effects on NGF secretion from C6 glioma cells. Compounds 3, 7, and 10 reduced NO levels in LPS-activated murine microglial cells with IC50 values of 26.89, 25.59, and 44.21 µM, respectively. Compounds 1, 5, and 9 upregulated NGF secretion to 153.09 ± 4.66, 156.88 ± 8.86, and 157.34 ± 3.30%, respectively.

Keyword

Impatiens balsamina; balsaminaceae; tetrahydronaphthalene; neuroprotective effect

MeSH Terms

Balsaminaceae
Chromatography
Glioma
Impatiens*
Inhibitory Concentration 50
Nerve Growth Factor
Neuroprotective Agents
Statistics as Topic
Nerve Growth Factor
Neuroprotective Agents

Figure

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  Fig. 1 Structures of compounds 1 – 12 isolated from I. balsamina

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  Fig. 2 Key 1H-1H COSY (―), HMBC (→) and NOESY (◂···▸) correlations of 1.

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