Ann Surg Treat Res.  2019 Jul;97(1):15-20. 10.4174/astr.2019.97.1.15.

The correlation between serum vascular endothelial growth factor (VEGF) and tumor VEGF receptor 3 in colorectal cancer

Affiliations
  • 1General Surgery, Hospital de Braga, Braga, Portugal. andre.b.goulart@gmail.com
  • 2Life and Health Science Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.

Abstract

PURPOSE
Despite plasma biomarkers offering a number of advantages over tissue-based markers, the relationship between serum vascular endothelial growth factor (VEGF) and VEGF receptor (VEGF-R) tumor expression in colorectal cancer (CRC) is still unclear. This study was designed to establish the relationship between the concentration of serum VEGF and tumor VEGF-R expression in patients with CRC.
METHODS
A prospective study of consecutive patients undergoing elective colorectal surgery during 1 year. Preoperative VEGF was determined by enzyme-linked immunosorbent assay and VEGF-R3 by immunochemistry.
RESULTS
The initial sample included 134 patients with CRC diagnosis. Results showed significant association of serum values of VEGF with VEGF-R3 expression (P < 0.001), even in the presence of confounders (sex, age, body mass index, tumor location, and surgical approach). The estimated effect size was high (η² = 0.35).
CONCLUSION
Serum VEGF has a significant correlation with tumoral VEGF-R3 expression in CRC.

Keyword

Colorectal neoplasms; Vascular endothelial growth factors; Enzyme-linked immunosorbent assay; Immunohistochemistry

MeSH Terms

Biomarkers
Body Mass Index
Colorectal Neoplasms*
Colorectal Surgery
Diagnosis
Enzyme-Linked Immunosorbent Assay
Humans
Immunochemistry
Immunohistochemistry
Plasma
Prospective Studies
Receptors, Vascular Endothelial Growth Factor*
Vascular Endothelial Growth Factor A*
Vascular Endothelial Growth Factors
Biomarkers
Receptors, Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors

Figure

  • Fig. 1 Immunohistochemical staining for VEGF-R3 in malignant colorectal endothelium. (A) Absent expression of VEGF-R3 in CRC (×100). (B) Weak/very limited moderate staining of VEGF-R3 (×100). (C) Moderate widespread/strong localized staining of VEGF-R3 (×100). (D) Strong widespread staining of VEGF-R3 (×100). VEGF, vascular endothelial growth factor; VEGF-R3, VEGF receptor 3; CRC, colorectal cancer.

  • Fig. 2 Serum values of VEGF distribution by immunochemistry VEGF-R3 expression. VEGF, vascular endothelial growth factor; VEGF-R3, VEGF receptor 3; CRC, colorectal cancer.


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