Infect Chemother.  2019 Jun;51(2):107-118. 10.3947/ic.2019.51.2.107.

Dissemination of Carbapenem-Resistance among Multidrug Resistant Pseudomonas aeruginosa carrying Metallo-Beta-Lactamase Genes, including the Novel bla(IMP-65) Gene in Thailand

Affiliations
  • 1Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. piyatip.khn@mahidol.edu
  • 2Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok, Thailand.

Abstract

BACKGROUND
Pseudomonas aeruginosa is considered as one of the most emerging threats in this century. Serious infections caused by this pathogen are often treated by carbapenems which are the last resource of antibiotics. Metallo-beta-lactamases (MBLs) production is one of the most important carbepenem resistance mechanisms and is usually related with nosocomial infections caused by P. aeruginosa. This study was aimed to determine the prevalence of MBL genes and distribution pattern of MBLs producing P. aeruginosa strains in Thailand.
MATERIALS AND METHODS
Specific primers were designed to detect MBL genes including IMP-, VIM-, and NDM-type MBL genes. Multilocus sequence typing method was used to determine the dissemination pattern of carbapenem-resistance among multidrug-resistant (CR-MDR) P. aeruginosa.
RESULTS
A total of 153 P. aeruginosa clinical isolates were characterized as CR-MDR. Among those, 31 P. aeruginosa clinical isolates (20.3%) presented metallo-beta-lactamase genes which could be divided into VIM-type (14 strains) and IMP-type (17 strains). bla IMP - 1, bla IMP - 13, bla IMP - 14a, and bla VIM - 2 genes were detected. Moreover, a novel IMP-type MBL, bla IMP - 65 was discovered and it was demonstrated to be the unique group of MBLs in Thailand. It was of interest that ST235 was the major ST type in Thailand followed by ST964 and ST111 and ST235 was detected in both MBL harboring and non-MBL harboring strains.
CONCLUSION
This study reported the dissemination of MBL gene including novel MBL, bla IMP-65. This study was also demonstrated major ST of P. aeruginosa which was ST235, followed by ST964 and ST111. Moreover, it is also the first report on many P. aeruginosa STs in Thailand: ST273, ST292, ST621, ST1584, and ST1816 which emphasized the dissemination trait difference of MBLs harboring P. aeruginosa in Thailand.

Keyword

Pseudomonas aeruginosa; Beta-lactamases; Multilocus sequence typing; Antibiotic resistance; ST235

MeSH Terms

Anti-Bacterial Agents
beta-Lactamases
Carbapenems
Cross Infection
Drug Resistance, Microbial
Methods
Multilocus Sequence Typing
Prevalence
Pseudomonas aeruginosa*
Pseudomonas*
Thailand*
Anti-Bacterial Agents
Carbapenems
beta-Lactamases
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