Tissue Eng Regen Med.  2019 Apr;16(2):161-175. 10.1007/s13770-019-00185-z.

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

• Koliakou I
• Gounari E
• Nerantzaki M
• Pavlidou E
• Bikiaris D
• Kaloyianni M
• Koliakos G

• KMID: 2449247
• DOI: http://doi.org/10.1007/s13770-019-00185-z

Abstract

BACKGROUND
Lonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films.
METHODS
For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO2ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells' genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton's Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay.
RESULTS
The highest differentiation capacity of MOMCs was observed on PCL/SiO2ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation.
CONCLUSION
To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO2ntbs or SrHAnrds into a polymeric matrix, for the first time.

Keyword

Monocyte-derived multipotential cells; Poly(e-caprolactone); Silica nanotubes; Strontium hydroxyapatite nanorods

MeSH Terms

Autografts
Beauty
Cell Line
Cell Lineage
Durapatite
Endothelium
Flow Cytometry
Human Umbilical Vein Endothelial Cells
Mesenchymal Stromal Cells
Methods
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Nanocomposites*
Nanotubes
Osteoblasts
Osteogenesis
Polymers
Real-Time Polymerase Chain Reaction
Regenerative Medicine
Silicon Dioxide
Strontium
Wharton Jelly
Durapatite
Polymers
Silicon Dioxide
Strontium