J Cancer Prev.  2019 Mar;24(1):54-58. 10.15430/JCP.2019.24.1.54.

Astaxanthin Prevents Decreases in Superoxide Dismutase 2 Level and Superoxide Dismutase Activity in Helicobacter pylori-infected Gastric Epithelial Cells

• Kim SH
• Lim JW
• Kim H

• KMID: 2447442
• DOI: http://doi.org/10.15430/JCP.2019.24.1.54

Abstract

BACKGROUND
Helicobacter pylori increases production of reactive oxygen species (ROS), which activates inflammatory and carcinogenesis-related signaling pathways in gastric epithelial cells. Therefore, reducing ROS, by upregulating antioxidant enzyme, such as superoxide dismutase (SOD), may be a novel strategy to prevent H. pylori-associated gastric diseases. Astaxanthin is an antioxidant carotenoid that prevents oxidative stress-induced cell injury. The present study was aimed to determine whether H. pylori decreases SOD activity by changing the levels of SOD1/SOD2 and whether astaxanthin prevents changes in SOD levels and activity in H. pylori-infected gastric epithelial AGS cells.
METHODS
AGS cells were pre-treated with astaxanthin for 3 hours prior to H. pylori infection and cultured for 1 hour in the presence of H. pylori. SOD levels and activity were assessed by Western blot analysis and a commercial assay kit, respectively. Mitochondrial ROS was determined using MitoSOX fluorescence.
RESULTS
H. pylori decreased SOD activity and the SOD2 level, but increased mitochondrial ROS in AGS cells. The SOD1 level was not changed by H. pylori infection. Astaxanthin prevented H. pylori-induced decreases in the SOD2 level and SOD activity and reduced mitochondrial ROS in AGS cells.
CONCLUSIONS
Consumption of astaxanthin-rich food may prevent the development of H. pylori-associated gastric disorders by suppressing mitochondrial oxidative stress.

Keyword

Astaxanthin; Helicobacter pylori; Gastric diseases; Reactive oxygen species; Superoxide dismutase

MeSH Terms

Blotting, Western
Epithelial Cells*
Fluorescence
Helicobacter pylori
Helicobacter*
Oxidative Stress
Reactive Oxygen Species
Stomach Diseases
Superoxide Dismutase*
Superoxides*
Reactive Oxygen Species
Superoxide Dismutase
Superoxides