Int J Oral Biol.  2019 Mar;44(1):20-26. 10.11620/IJOB.2019.44.1.20.

Porphyromonas gingivalis lipopolysaccharide stimulates vascular smooth muscle cell migration through signal transducer and activator of transcription 3-mediated matrix metalloproteinase-9 expression

• Kim Y
• Park JY
• Park HJ
• Kim MK
• Kim YI
• Bae SK
• Kim HJ
• Bae MK

• KMID: 2446937
• DOI: http://doi.org/10.11620/IJOB.2019.44.1.20

Abstract

Periodontal diseases have been associated with the development of cardiovascular diseases. Accumulating evidences have indicated that Porphyromonas gingivalis, a major periodontopathic pathogen, plays a critical role in the pathogenesis of atherosclerosis. In the present study, we demonstrated that P. gingivalis lipopolysaccharide (LPS) increases the mRNA and protein expression of matrix metalloproteinase-9 (MMP-9) in rat vascular smooth muscle cells. We showed that the MMP-9 expression induced by P. gingivalis LPS is mediated by the activation of signal transducer and activator of transcription 3 (STAT3) in vascular smooth muscle cells. Furthermore, the inhibition of STAT3 activity reduced P. gingivalis LPS-induced migration of vascular smooth muscle cells. Overall, our findings indicate that P. gingivalis LPS stimulates the migration of vascular smooth muscle cells via STAT3-mediated MMP-9 expression.

Keyword

Porphyromonas gingivalis; Vascular smooth muscle cells; Matrix metalloproteinase 9; STAT3 transcription factor

MeSH Terms

Animals
Atherosclerosis
Cardiovascular Diseases
Cell Movement*
Matrix Metalloproteinase 9*
Muscle, Smooth, Vascular*
Periodontal Diseases
Porphyromonas gingivalis*
Porphyromonas*
Rats
RNA, Messenger
STAT3 Transcription Factor
Transducers*
Matrix Metalloproteinase 9
RNA, Messenger
STAT3 Transcription Factor