World J Mens Health.  2019 May;37(2):249-256. 10.5534/wjmh.180105.

Protective Effect of All-Trans Retinoic Acid in Cisplatin-Induced Testicular Damage in Rats

• Yucel C
• Arslan FD
• Ekmekci S
• Ulker V
• Kisa E
• Erdogan Yucel E
• Ucar M
• Ilbey YO
• Celik O
• Basok BI
• Kozacioglu Z

• KMID: 2443241
• DOI: http://doi.org/10.5534/wjmh.180105

Abstract

PURPOSE
To investigate the effects of all-trans retinoic acid (ATRA) in cisplatin (CP)-induced testicular damage in rats.
MATERIALS AND METHODS
Twenty-eight male Wistar rats were divided into four groups: Control, ATRA alone, ATRA+CP, and CP alone. Body weight, testicular weight, sperm count, sperm motility, percentage of abnormal sperm, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) in testicular tissue, and testicular histopathology were compared among groups.
RESULTS
The sperm count and motility significantly decreased and the percentage of abnormal sperm significantly increased in the CP group compared to the control and ATRA groups. CP+ATRA administration significantly increased the sperm count and motility, but reduced the abnormal sperm count. CP administration significantly increased TOS and OSI compared to the control group and the other groups. Administering CP+ATRA significantly decreased TOS and the OSI in testicular tissue and reduced spermatogenesis, but increased the Johnsen score.
CONCLUSIONS
The destructive effects of CP treatment on testicular tissue and spermatogenesis were reduced by administering ATRA.

Keyword

Cisplatin; Oxidative stress; Spermatogenesis; Testis; Vitamin A

MeSH Terms

Animals
Body Weight
Cisplatin
Humans
Male
Oxidative Stress
Rats*
Rats, Wistar
Sperm Count
Sperm Motility
Spermatogenesis
Spermatozoa
Testis
Tretinoin*
Vitamin A
Cisplatin
Tretinoin
Vitamin A

Figure

• 

  Fig. 1 Box graphics of sperm count (A), motility (B), and abnormal sperm rate (C). ATRA: all-trans retinoic acid, CP: cisplatin.

• 

  Fig. 2 Box graphics of total antioxidant status (TAS) (A), total oxidant status (TOS) (B), and oxidative stress index (OSI) (C). ATRA: all-trans retinoic acid, CP: cisplatin.

• 

  Fig. 3 Testicular histopathology in control group (A), all-trans retinoic acid+cisplatin (CP) group (B), and CP group (C) (A–C: H&E, ×400).

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