Korean J Parasitol.  2018 Dec;56(6):553-558. 10.3347/kjp.2018.56.6.553.

Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii

• Moon EK
• Choi HS
• Park SM
• Kong HH
• Quan FS

• KMID: 2441615
• DOI: http://doi.org/10.3347/kjp.2018.56.6.553

Abstract

Pathogenic Acanthamoeba spp. cause granulomatous amoebic encephalitis and keratitis. Acanthamoeba keratitis (AK) is a rare but serious ocular infection that can result in permanent visual impairment or blindness. However, pathogenic factors of AK remain unclear and treatment for AK is arduous. Expression levels of proteins secreted into extracellular space were compared between A. castellanii pathogenic (ACP) and non-pathogenic strains. Two-dimensional polyacrylamide gel electrophoresis revealed 123 differentially expressed proteins, including 34 increased proteins, 7 qualitative increased proteins, 65 decreased proteins, and 17 qualitative decreased proteins in ACP strain. Twenty protein spots with greater than 5-fold increase in ACP strain were analyzed by liquid chromatography triple quadrupole mass spectrometry. These proteins showed similarity each to inosine-uridine preferring nucleoside hydrolase, carboxylesterase, oxygen-dependent choline dehydrogenase, periplasmic-binding protein proteinases and hypothetical proteins. These proteins expressed higher in ACP may provide some information to understand pathogenicity of Acanthamoeba.

Keyword

Acanthamoeba castellanii; secretory product

MeSH Terms

Acanthamoeba castellanii*
Acanthamoeba Keratitis
Acanthamoeba*
Blindness
Carboxylesterase
Choline Dehydrogenase
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Encephalitis
Extracellular Space*
Eye Infections
Keratitis
Mass Spectrometry
Peptide Hydrolases
Virulence
Vision Disorders
Carboxylesterase
Choline Dehydrogenase
Peptide Hydrolases