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Contamination of Acanthamoeba in Contact Lens Care System

Lee SM, Choi YJ, Chung DI

  • KMID: 2147740
  • J Korean Ophthalmol Soc.
  • 1997 May;38(5):756-761.
Recently, the number of reports of Acanthamoeba keratitis has been increased apparently as a consequence of association with use of contact lenses, contaminated contact lens care system and high concern...
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In vivo tandem scanning confocal microscopy in acanthamoeba keratitis

Cho BJ, Holland EJ

The in vivo confocal microscopy technique provides us with a real-time, non-invasive way of examining the human cornea. The most important advantage of this type of microscopy is to reveal...
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The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea

Jeong HJ, Yu HS

A survey was carried out from August to December 2004 in Pusan, Korea to document the presence of free-living amoeba (FLA), including the genus Acanthamoeba, in both contact lens storage...
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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoeba sp. YM-4 (Korean isolate)

Shin HJ, Im K, Jeon KW

Acanthamoeba sp. YM-4 is similar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in vitro cytotoxicity and isoenzyme patterns....
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Evaluation of Three Different Methods to Establish Animal Models of Acanthamoeba Keratitis

Ren M, Wu X

PURPOSE: To produce animal models of Acanthamoeba keratitis and to evaluate the advantages and adaptation range of each of the three methods employed. MATERIALS AND METHODS: Mice and Wistar rats...
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Comparison of Usefulness of Laboratory Diagnosis in Ancanthamoeba Keratitis

Hahn TW, Hahn YH

  • KMID: 2205341
  • J Korean Ophthalmol Soc.
  • 1998 Oct;39(10):2218-2225.
Laboratory diagnostic methods, applied for the diagnosis of Acanthamoeba Keratitis, were evaluated for their usefulness in 16 patients of suspicious Acanthamoeba keratitis. Wet smear, Acridine orange(AO) stain, Gram stain...
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Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish

Im KI, Shin HJ

A new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Acanthamoeba sp. YM-4 (Korean isolate YM-4). The trophozoites were 11.0-23.0 micrometer in length and...
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Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates

Lee JE, Park JS, Lee JS, Yoo HS

  • KMID: 2126716
  • J Korean Ophthalmol Soc.
  • 2007 Mar;48(3):431-437.
PURPOSE: To evaluate the cysticidal effect of 5 kinds of commercially available contact lens disinfectants against 2 clinical isolates of Acanthamoeba. METHODS: Five kinds of commercially available contact lens disinfectants were...
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Two Cases of Corneal Toxicity in Acanthamoeba Keratitis by Combined Topical Anti-Acanthamoeba Keratitis Eye Solution

Lee JK, Lee JS

PURPOSE: To present a case of corneal toxicity caused by therapeutic duplication during treatment of acanthamoeba keratitis as a complication of contact lens use. CASE SUMMARY: A 12-year-old girl with a...
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Acanthamoeba Keratitis Related to Orthokeratology Contact Lens

Lee JE, Hahn TW, Yu HS, Lee JS

  • KMID: 2337493
  • J Korean Ophthalmol Soc.
  • 2007 Feb;48(2):328-331.
PURPOSE: To report 4 cases of Acanthamoeba keratitis related to orthokeratology lens overnight wear. METHODS: Four patients had histories of overnight orthkeratology lens wear of 10 months to 3 years when...
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In vitro cytotoxicity of Acanthamoeba spp. isolated from contact lens containers in Korea by crystal violet staining and LDH release assay

Shin HJ, Cho MS, Jung SY, Kim HI, Im KI

In order to observe the cytotoxicity of Acanthamoeba spp., which were isolated from contact lens containers as ethiological agents for the probable amoebic keratitis in Korea, the crystal violet staining...
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Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba

Moon EK, Kong HH

  • KMID: 1792132
  • Korean J Parasitol.
  • 2012 Dec;50(4):361-364.
The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose...
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Degradation of immunoglobulins, protease inhibitors, and interleukin-1 by a secretory proteinase of Acanthamoeba castellanii

Na BK, Cho JH, Song CY, Kim TS

The effect of a secretory proteinase from the pathogenic amoebae Acanthamoeba castellanii on hosts defense-oriented or regulatory proteins such as immunoglobulins, interleukin-1, and protease inhibitors was investigated. The enzyme was...
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Ultrastructural changes of Acanthamoeba cyst of clinical isolates after treatment with minimal cysticidal concentration of polyhexamethylene biguanide

Kong HH, Chung DI

In order to understand the action mechanism of polyhexamethylene biguanide (PHMB) to the cyst of Acanthamoeba on the morphological basis, the cysts of four corneal isolates of Acanthamoeba were treated...
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Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China

Wang M, Sun G, Sun Y, You X, Li X, Cheng Y, Xuan Y

Members of genus Acanthamoeba are widely distributed in the environment. Some are pathogenic and cause keratitis and fatal granulomatous amoebic encephalitis. In this study, we isolated an Acanthamoeba CJW/W1 strain...
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Isoenzyme patterns and phylogenetic relationships in Acanthamoeba spp. isolated from contact lens containers in Korea

Shin HJ, Cho MS, Kim HJ, Im KI

In order to refer to the basic information regarding the identification of isolates obtained from a contact lens container in Korea, the isoelectric focusing gel electrophoresis was employed to...
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Construction of EST Database for Comparative Gene Studies of Acanthamoeba

Moon EK, Kim JO, Xuan YH, Yun YS, Kang SW, Lee YS, Ahn TI, Hong YC, Chung DI, Kong HH

The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba...
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Pathogenic free-living amoebae in Korea

Shin HJ, Im KI

Acanthamoeba and Naegleria are widely distributed in fresh water, soil and dust throughout the world, and cause meningoencephalitis or keratoconjunctivitis in humans and other mammals. Korean isolates, namely, Naegleria sp....
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Pathogenicity of Korean isolates of Acanthamoeba by observing the experimental infection and zymodemes of five isoenzymes

Im KI, Seo DW, Jeon SH, Kim TE

To determine the pathogenicity of Acanthamoeba spp. isolated in Korea and to develop a isoenzymatic maker, the mortality rate of infected mice, in vitro cytotoxicity against target cells and isoenzyme...
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Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii

Moon EK, Park HR, Quan FS, Kong HH

Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing...
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