J Biomed Transl Res.  2018 Mar;19(1):10-15. 10.12729/jbtr.2018.19.1.010.

Expression of p63 and its association with cell proliferation at different stages of murine hair follicle cycle

  • 1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea. byoon@kangwon.ac.kr
  • 2Biotoxtech Co., Cheongju 28115, Korea.
  • 3Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA, Wanju 55365, Korea.


In the present study, we investigated the expression patterns of p63, a member of the p53 gene family, in hair follicle cells at different stages of the hair cycle and examined the relation with cell proliferation activity. For this study, immunohistochemistry for p63 and Ki-67, a marker of cell proliferation, was performed in skin obtained from C3H/he mice with depilation. In the anagen stage, p63 was strongly expressed in the cells of bulge areas and epithelial strand, matrix cells of the hair bulbs and outer root sheath cells, but inner root sheath cells and dermal papilla cells were negative for p63. These expression patterns of p63 were similarly noted in hair follicles in the early catagen stage. In the late catagen and telogen stages of hair follicles, outer root sheath cells, seboblasts and duct cells were immunoreactive for p63. On the other hand, Ki-67-positive cells were selectively observed among the p63 positive cell components, although p63 positive cells were not always proliferative. Most of the matrix cells in the hair bulbs were positive for Ki-67. Ki-67-positive cells were also frequently evident in the cells of epithelial strands in the early anagen stage. Outer root sheath cells were often positive for Ki-67 in the anagen and early catagen stages, but very rare in the late catagen and telogen stages. In summary, p63 was expressed in the bulge stem cells, epithelial strand cells, matrix cells and outer root sheath cells of hair follicles at any stage of the cycle, which was associated with the movement of hair progenitor cells for regeneration. Ki-67-positive cells were evident among the p63-expressing cell components. Our results strongly suggest that p63 plays an important role in stem cell regulation, at least associated with cell proliferation, for the regeneration of hair follicles.


hair follicle cycle; immunohistochemistry; Ki-67; mouse; p63

MeSH Terms

Cell Proliferation*
Cellular Structures
Genes, p53
Hair Follicle*
Hair Removal
Stem Cells
Full Text Links
  • JBTR
export Copy
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr