Ann Lab Med.  2018 Nov;38(6):518-523. 10.3343/alm.2018.38.6.518.

Removing Lipemia in Serum/Plasma Samples: A Multicenter Study

Affiliations
  • 1Clinical Laboratory, Bellvitge University Hospital, Barcelona, Spain. mjcastro@bellvitgehospital.cat
  • 2Biochemitry Service, Cruces University Hospital, Vizcaya, Spain.
  • 3Laboratory of Clinical Biochemistry, Miguel Servet University Hospital, Zaragoza, Spain.
  • 4Laboratory of Clinical Biochemistry, Virgen del Rocío University Hospital, Sevilla, Spain.
  • 5Laboratory of Clinical Biochemistry, Albacete Hospital, Albacete, Spain.
  • 6Department of Biochemistry and Molecular and Cellular Biology, University of Zaragoza, Zaragoza, Spain.
  • 7Laboratory of Clinical Biochemistry, Gran Canaria Dr. Negrín University Hospital, Las Palmas de Gran Canaria, Spain.

Abstract

BACKGROUND
Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method.
METHODS
Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods"”LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI).
RESULTS
When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI.
CONCLUSIONS
High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.

Keyword

Lipemia; Interference; Lipid removal method; High-speed centrifugation; LipoClear; 1,1,2-trichlorotrifluoroethane

MeSH Terms

Alanine Transaminase
Alkaline Phosphatase
Aspartate Aminotransferases
Bilirubin
Calcium
Centrifugation
Creatinine
Glucose
Hyperlipidemias*
Laboratories, Hospital
Liquid-Liquid Extraction
Methods
Potassium
Sodium
Spain
Transferases
Ultracentrifugation
Urea
Alanine Transaminase
Alkaline Phosphatase
Aspartate Aminotransferases
Bilirubin
Calcium
Creatinine
Glucose
Potassium
Sodium
Transferases
Urea

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