Biomol Ther.  2018 Mar;26(2):218-223. 10.4062/biomolther.2017.199.

Hepaprotective Effect of Standardized Ecklonia stolonifera Formulation on CClâ‚„-Induced Liver Injury in Sprague-Dawley Rats

• Byun JH
• Kim J
• Choung SY

• KMID: 2429062
• DOI: http://doi.org/10.4062/biomolther.2017.199

Abstract

The liver is an essential organ for the detoxification of exogenous xenobiotics, drugs and toxic substances. The incidence rate of non-alcoholic liver injury increases due to dietary habit change and drug use increase. Our previous study demonstrated that Ecklonia stolonifera (ES) formulation has hepatoprotective effect against alcohol-induced liver injury in rat and tacrine-induced hepatotoxicity in HepG2 cells. This present study was designated to elucidate hepatoprotective effects of ES formulation against carbon tetrachloride (CClâ‚„)-induced liver injury in Sprague Dawley rat. Sixty rats were randomly divided into six groups. The rats were treated orally with ES formulation and silymarin (served as positive control, only 100 mg/kg/day) at a dose of 50, 100, or 200 mg/kg/day for 21 days. Seven days after treatment, liver injury was induced by intraperitoneal injection of CClâ‚„ (1.5 ml/kg, twice a week for 14 days). The administration of CClâ‚„ exhibited significant elevation of hepatic enzymes (like AST and ALT), and decrease of antioxidant related enzymes (superoxide dismutase, glutathione peroxidase and catalase) and glutathione. Then, it leaded to DNA damages (8-oxo-2"²-deoxyguanosine) and lipid peroxidation (malondialdehyde). Administration of ES formulation inhibited imbalance of above factors compared to CClâ‚„ induced rat in a dose dependent manner. Real time PCR analysis indicates that CYP2E1 was upregulated in CClâ‚„ induced rat. However, increased gene expression was compromised by ES formulation treatment. These findings suggests that ES formulation could protect hepatotoxicity caused by CClâ‚„ via two pathways: elevation of antioxidant enzymes and normalization of CYP2E1 enzyme.

Keyword

Ecklonia stolonifera; Hepatoprotective effect; Non-alcoholic liver injury; CYP2E1; Antioxidant enzymes; Carbon tetrachloride

MeSH Terms

Animals
Carbon Tetrachloride
Cytochrome P-450 CYP2E1
DNA Damage
Food Habits
Gene Expression
Glutathione
Glutathione Peroxidase
Hep G2 Cells
Incidence
Injections, Intraperitoneal
Lipid Peroxidation
Liver*
Rats
Rats, Sprague-Dawley*
Real-Time Polymerase Chain Reaction
Silymarin
Xenobiotics
Carbon Tetrachloride
Cytochrome P-450 CYP2E1
Glutathione
Glutathione Peroxidase
Silymarin
Xenobiotics