Arch Plast Surg.  2018 Nov;45(6):504-511. 10.5999/aps.2018.00948.

Wound healing effects of paste type acellular dermal matrix subcutaneous injection

• Lee JH
• Kim JW
• Lee JH
• Chung KJ
• Kim TG
• Kim YH
• Kim KJ

• KMID: 2427539
• DOI: http://doi.org/10.5999/aps.2018.00948

Abstract

BACKGROUND
Acellular dermal matrix (ADM) helps wound healing by stimulating angiogenesis, acting as a chemoattractant for endothelial cells, providing growth factors, and permitting a substrate for fibroblasts to attach. The current standard for using paste-type ADM (CG Paste) in wound healing is direct application over the wounds. The major concerns regarding this method are unpredictable separation from the wounds and absorption into negative-pressure wound therapy devices. This study aimed to investigate the effects of subcutaneous injection of paste-type ADM on wound healing in rats.
METHODS
Full-thickness skin defects were created on the dorsal skin of rats. Eighteen rats were randomly divided into three groups and treated using different wound coverage methods: group A, with a saline dressing; group B, standard application of CG Paste; and group C, injection of CG Paste. On postoperative days 3, 5, 7, 10, and 14, the wound areas were analyzed morphologically. Histological and immunohistochemical tissue analyses were performed on postoperative days 3 and 7.
RESULTS
Groups B and C had significantly less raw surface than group A on postoperative days 10 and 14. Collagen fiber deposition and microvessel density were significantly higher in group C than in groups A and B on postoperative days 3 and 7.
CONCLUSIONS
This study showed comparable effectiveness between subcutaneous injection and the conventional dressing method of paste-type ADM. Moreover, the injection of CG Paste led to improved wound healing quality through the accumulation of collagen fibers and an increase in microvessel density.

Keyword

Wound healing; Ointments; Acellular dermis

MeSH Terms

Absorption
Acellular Dermis*
Animals
Bandages
Collagen
Endothelial Cells
Fibroblasts
Injections, Subcutaneous*
Intercellular Signaling Peptides and Proteins
Methods
Microvessels
Negative-Pressure Wound Therapy
Ointments
Rats
Skin
Wound Healing*
Wounds and Injuries*
Collagen
Intercellular Signaling Peptides and Proteins
Ointments