Biomol Ther.  2018 Nov;26(6):539-545. 10.4062/biomolther.2018.006.

Suppressive Effect of 4-Hydroxy-2-(4-Hydroxyphenethyl) Isoindoline-1,3-Dione on Ovalbumin-Induced Allergic Asthma

• Huang J
• Su M
• Lee BK
• Kim MJ
• Jung JH
• Im DS

• KMID: 2427506
• DOI: http://doi.org/10.4062/biomolther.2018.006

Abstract

4-Hydroxy-2-(4-hydroxyphenethyl)isoindoline-1,3-dione (PD1) is a synthetic phthalimide derivative of a marine compound. PD1 has peroxisome proliferator-activated receptor (PPAR) γ agonistic and anti-inflammatory effects. This study aimed to investigate the effect of PD1 on allergic asthma using rat basophilic leukemia (RBL)-2H3 mast cells and an ovalbumin (OVA)-induced asthma mouse model. In vitro, PD1 suppressed β-hexosaminidase activity in RBL-2H3 cells. In the OVA-induced allergic asthma mouse model, increased inflammatory cells and elevated Th2 and Th1 cytokine levels were observed in bronchoalveolar lavage fluid (BALF) and lung tissue. PD1 administration decreased the numbers of inflammatory cells, especially eosinophils, and reduced the mRNA and protein levels of the Th2 cytokines including interleukin (IL)-4 and IL-13, in BALF and lung tissue. The severity of inflammation and mucin secretion in the lungs of PD1-treated mice was also less. These findings indicate that PD1 could be a potential compound for anti-allergic therapy.

Keyword

PD1; Anti-allergic; Anti-asthma; Ovalbumin; RBL-2H3

MeSH Terms

Animals
Asthma*
Basophils
Bronchoalveolar Lavage Fluid
Cytokines
Eosinophils
In Vitro Techniques
Inflammation
Interleukin-13
Interleukins
Leukemia
Lung
Mast Cells
Mice
Mucins
Ovalbumin
Peroxisomes
Rats
RNA, Messenger
Cytokines
Interleukin-13
Interleukins
Mucins
Ovalbumin
RNA, Messenger