Clin Exp Emerg Med.
2018 Sep;5(3):192-198. 10.15441/ceem.17.253.
Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats
- Affiliations
-
- 1Department of Emergency Medicine, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea.
- 2Institute of Health Sciences, Gyeongsang National University, Jinju, Korea. gnuhpis@gnu.ac.kr
- 3Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Korea.
- 4Department of Emergency Medicine, Catholic University of Daegu, Daegu, Korea.
- 5Department of Neurosurgery, Gyeongsang National University Hospital, Gyeongsang National University School of Medicine, Jinju, Korea.
- KMID: 2422662
- DOI: http://doi.org/10.15441/ceem.17.253
Abstract
OBJECTIVE
Dapsone (diaminodiphenyl sulfone, DDS) is currently used to treat leprosy, malaria, dermatitis herpetiformis, and other diseases. It is also used to treat pneumocystis pneumonia and Toxoplasma gondii infection in HIV-positive patients. The most common adverse effect of DDS is methemoglobinemia from oxidative stress. Ascorbic acid is an antioxidant and reducing agent that scavenges the free radicals produced by oxidative stress. The present study aimed to investigate the effect of ascorbic acid in the treatment of DDS induced methemoglobinemia.
METHODS
Male Sprague-Dawley rats were divided into three groups: an ascorbic acid group, a methylene blue (MB) group, and a control group. After DDS (40 mg/kg) treatment via oral gavage, ascorbic acid (15 mg/kg), MB (1 mg/kg), or normal saline were administered via tail vein injection. Depending on the duration of the DDS treatment, blood methemoglobin levels, as well as the nitric oxide levels and catalase activity, were measured at 60, 120, or 180 minutes after DDS administration.
RESULTS
Methemoglobin concentrations in the ascorbic acid and MB groups were significantly lower compared to those in the control group across multiple time points. The plasma nitric oxide levels and catalase activity were not different among the groups or time points.
CONCLUSION
Intravenous ascorbic acid administration is effective in treating DDS-induced methemoglobinemia in a murine model.
Keyword
MeSH Terms
-
Animals
Ascorbic Acid*
Catalase
Dapsone
Dermatitis Herpetiformis
Free Radicals
Humans
Leprosy
Malaria
Male
Methemoglobin
Methemoglobinemia*
Methylene Blue
Nitric Oxide
Oxidative Stress
Plasma
Pneumonia, Pneumocystis
Rats*
Rats, Sprague-Dawley
Tail
Toxoplasmosis
Veins
Ascorbic Acid
Catalase
Dapsone
Free Radicals
Methemoglobin
Methylene Blue
Nitric Oxide
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