Biomol Ther.  2018 May;26(3):290-297. 10.4062/biomolther.2017.240.

Construction of a Transcriptome-Driven Network at the Early Stage of Infection with Influenza A H1N1 in Human Lung Alveolar Epithelial Cells

• Chung M
• Cho SY
• Lee YS

• KMID: 2421738
• DOI: http://doi.org/10.4062/biomolther.2017.240

Abstract

We aimed to understand the molecular changes in host cells that accompany infection by the seasonal influenza A H1N1 virus because the initial response rapidly changes owing to the fact that the virus has a robust initial propagation phase. Human epithelial alveolar A549 cells were infected and total RNA was extracted at 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and 48 h post infection (h.p.i.). The differentially expressed host genes were clustered into two distinct sets of genes as the infection progressed over time. The patterns of expression were significantly different at the early stages of infection. One of the responses showed roles similar to those associated with the enrichment gene sets to known "˜gp120 pathway in HIV.' This gene set contains genes known to play roles in preventing the progress of apoptosis, which infected cells undergo as a response to viral infection. The other gene set showed enrichment of "˜Drug Metabolism Enzymes (DMEs).' The identification of two distinct gene sets indicates that the virus regulates the cell's mechanisms to create a favorable environment for its stable replication and protection of gene metabolites within 8 h.

Keyword

A549 Cells; Influenza A virus H1N1 subtype; High-throughput nucleotide sequencing; Sequence analysis RNA; Apoptosis; Gene expression regulation

MeSH Terms

Apoptosis
Epithelial Cells*
Gene Expression Regulation
High-Throughput Nucleotide Sequencing
Humans*
Influenza A Virus, H1N1 Subtype
Influenza, Human*
Lung*
Metabolism
RNA
Seasons
RNA