Endocrinol Metab.  2018 Jun;33(2):245-251. 10.3803/EnM.2018.33.2.245.

Distinct Ultradian Rhythms in Plasma Clusterin Concentrations in Lean and Obese Korean Subjects

• Choi JH
• Jeong E
• Youn BS
• Kim MS

• KMID: 2420493
• DOI: http://doi.org/10.3803/EnM.2018.33.2.245

Abstract

BACKGROUND
Blood levels of many hormones show rhythmic fluctuations with variable duration of cycles. Clusterin/apolipoprotein J is a glycoprotein which is highly expressed in the plasma and has modulatory roles in immune and inflammatory reactions, neurobiology, lipid metabolism, and leptin signaling. In this study, we examined the diurnal fluctuations of plasma clusterin concentrations in lean and obese young men.
METHODS
For the study, 14 subjects (five lean and five obese men; two lean and two obese women) were admitted to the research ward and blood samples were drawn every 30 minutes during light-on period (6:00 AM to 10:00 PM) and every hour during light-off period.
RESULTS
Notably, plasma clusterin concentrations displayed a unique ultradian rhythm with five cycles a day in both men and women. During the light-on period, circulating clusterin levels showed fluctuating curves with 4 hours regular intervals with sharp peaks and troughs. In contrast, single oscillation curve during light-off exhibited a smoothened/lower peak and longer (8-hour) duration. In obese men, these cycles were phase-advanced by approximately 1 hour, and had reduced amplitude of fluctuating curves and blunted diurnal pattern. Cyclic fluctuations of plasma clusterin were preserved under fasting and unexpected meal condition, suggesting that rhythmic oscillations in plasma clusterin levels are not generated by meal-related cues.
CONCLUSION
These findings firstly demonstrate a novel pattern of plasma clusterin fluctuations with extremely regular cycles.

Keyword

Clusterin; Obesity; Ultradian rhythm; Circadian rhythm

MeSH Terms

Circadian Rhythm*
Clusterin*
Cues
Fasting
Female
Glycoproteins
Humans
Leptin
Lipid Metabolism
Male
Meals
Neurobiology
Obesity
Plasma*
Clusterin
Glycoproteins
Leptin

Figure

• 

  Fig. 1 Ultradian rhythms in plasma clusterin concentrations of (A) total subjects (n=10) and (B) lean and obese subjects (n=5 per group). P, peak; T, trough; C, cycle; B, breakfast; L, lunch; D, dinner.

• 

  Fig. 2 Ultradian rhythms in plasma clusterin concentrations of four woman subjects (two lean and two obese). aObese 1: age 28 years, body mass index (BMI) 32.9; bObese 2: age 28 years, BMI 30.4; cLean 1: age 31 years, BMI 19.1; dLean 2: age 23 years, BMI 18.9.

• 

  Fig. 3 Comparison of plasma clusterin diurnal fluctuations between regular three meal condition and fasting-unexpected meal condition (n=4).

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