Obstet Gynecol Sci.  2018 Jan;61(1):127-134. 10.5468/ogs.2018.61.1.127.

The expression of Müllerian inhibiting substance/anti-Müllerian hormone type II receptor in myoma and adenomyosis

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea. janghkim@catholic.ac.kr

Abstract


OBJECTIVE
We compared the expression levels of Müllerian inhibiting substance (MIS)/anti-Müllerian hormone type II receptor (AMHRII) in uterine myoma and adenomyosis to evaluate the possibility of using MIS/anti-Müllerian hormone (AMH) as a biological regulator or therapeutic agent in patients with uterine leiomyoma and adenomyosis.
METHODS
We studied normal uterine myometrium, leiomyoma, endometrial tissue, and adenomyosis from 57 patients who underwent hysterectomy for uterine leiomyoma (22 cases) or adenomyosis (28 cases) and myomectomy for uterine myoma (7 cases). Immunohistochemical staining was used to confirm the MIS/AMHRII protein expression level in each tissue. Reverse transcription-polymerase chain reaction was performed to quantify MIS/AMHRII mRNA expression.
RESULTS
The MIS/AMHRII protein was more strongly expressed in uterine myoma (frequency of MIS/AMHRII expressing cells: 51.95%±13.96%) and adenomyosis (64.65%±4.85%) tissues than that in the normal uterine myometrium (3.15%±1.69%) and endometrium (31.10%±7.19%). In the quantitative analysis of MIS/AMHRII mRNA expression, MIS/AMHRII mRNA expression levels in uterine myoma (mean density: 4.51±0.26) and adenomyosis (6.84±0.20) tissues were higher than that in normal uterine myometrial tissue (0.08±0.09) and endometrial tissue (1.63±0.06).
CONCLUSION
This study demonstrated that MIS/AMHRII was highly and strongly expressed on uterine myoma and adenomyosis. Our data suggest that MIS/AMH may be evaluated as a biological modulator or therapeutic agent on MIS/AMHRII expressing uterine myoma and adenomyosis.

Keyword

Adenomyosis; Anti-Müllerian hormone; Anti-Müllerian hormone receptor; Myoma

MeSH Terms

Adenomyosis*
Animals
Endometrium
Female
Humans
Hysterectomy
Leiomyoma
Mice
Myoma*
Myometrium
RNA, Messenger
RNA, Messenger

Figure

  • Fig. 1 (A) Reverse transcription-polymerase chain reaction (RT-PCR) results for human Müllerian inhibiting substance type II receptor (MISRII) from human (a) myometrium, (b) myoma, (c) endometrium, and (d) adenomyosis. (B) Quantification of RT-PCR signals obtained using densitometric analysis of the signal product optical density×resulting band area.GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

  • Fig. 2 Light photomicrographs of human (A) liver (negative control), (B) granulosa cells of preantral follicle in the ovary (positive control), (C) myometrium, (D) myoma, (E) endometrium, and (F) adenomyosis. Each image shows the expression of Müllerian inhibiting substance/anti-Müllerian hormone type II receptor (MIS/AMHRII) in the cell membrane. Right lower boxed area is of higher magnification (×400). Chromogen, amino ethyl carbazol. Magnification (×200).

  • Fig. 3 The intensity score for Müllerian hormone type II receptor (MISRII) expression in (A) myometrium, (B) myoma, (C) endometrium, and (D) adenomyosis.a)P<0.001, myometrium vs. myoma; b)P<0.05, endometrium vs. adenomyosis.


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