Yonsei Med J.  2017 Nov;58(6):1092-1100. 10.3349/ymj.2017.58.6.1092.

Knockdown of Long Non-Coding RNA NEAT1 Inhibits Proliferation and Invasion and Induces Apoptosis of Osteosarcoma by Inhibiting miR-194 Expression

Affiliations
  • 1Department of Orthopedics, Zhoukou Central Hospital, Zhoukou, China. wangheping112@yeah.net
  • 2Department of Surgery, Zhoukou Central Hospital, Zhoukou, China.

Abstract

PURPOSE
Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma.
MATERIALS AND METHODS
Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively. Luciferase reporter assay was performed to observe the possible interaction between NEAT1 and miR-194.
RESULTS
NEAT1 was upregulated and miR-194 was downregulated in osteosarcoma tissues and cells. Knockdown of NEAT1 or overexpression of miR-194 suppressed proliferation and invasion and induced apoptosis of osteosarcoma cells in vitro. Luciferase reporter assay validated that NEAT1 could interact with miR-194 and negatively modulated its expression. Furthermore, inhibition of miR-194 reversed the suppression of proliferation and invasion and the promotion of apoptosis induced by NEAT1 depletion in osteosarcoma cells.
CONCLUSION
Knockdown of NEAT1 suppressed proliferation and invasion and induced apoptosis in osteosarcoma cells by inhibiting miR-194 expression.

Keyword

lncRNA; tumorigenesis; osteosarcoma; miR-194

MeSH Terms

Apoptosis/*genetics
Bone Neoplasms/genetics
Cell Proliferation
Disease Progression
Down-Regulation
*Gene Expression Regulation, Neoplastic
Humans
MicroRNAs/*genetics/metabolism
Osteosarcoma/*genetics/pathology
RNA, Long Noncoding/*genetics
Real-Time Polymerase Chain Reaction
Up-Regulation
MicroRNAs
RNA, Long Noncoding
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