Clin Exp Vaccine Res.  2018 Jul;7(2):139-144. 10.7774/cevr.2018.7.2.139.

Genetic identification and serological evaluation of commercial inactivated foot-and-mouth disease virus vaccine in pigs

Affiliations
  • 1College of Veterinary Medicine, Konkuk University, Seoul, Korea. lyoo@konkuk.ac.kr
  • 2Hipra Korea Inc., Seongnam, Korea.

Abstract

Vaccination is considered a frequently used tool to prevent and control foot-and-mouth disease (FMD). However, the effectiveness of conventional FMD virus (FMDV) vaccines in pigs has been controversial because the massive prophylactic vaccination could not elicit proper immune response nor prevent the broad spread of FMD outbreak, mainly in pig farms, in South Korea during outbreaks of 2014. In addition, there has been little information on the efficacy of inactivated, high potency, multivalent, oil-based FMDV vaccine in pigs, because an evaluation of FMDV vaccines had been mainly carried out using cattle. In this study, we evaluated the genetic identification of commercial inactivated FMDV vaccine and monitored the immune responses in pigs under the field condition. Results implied that it contained three different serotypes with a high level of antigen payload. However, serological results showed low mean percentage of inhibition, and positive rate reached its peak at 6-week post-vaccination, indicating current FMDV vaccine need to improve for a prophylactic vaccination policy in pigs. Therefore, there is an imperative need to develop FMDV vaccine that can provide rapid and long-lasting protective immunity in pigs.

Keyword

Antibody formation; Foot-and-mouth disease; Real-time polymerase chain reaction; Vaccines

MeSH Terms

Agriculture
Animals
Antibody Formation
Cattle
Disease Outbreaks
Foot-and-Mouth Disease Virus*
Foot-and-Mouth Disease*
Korea
Real-Time Polymerase Chain Reaction
Serogroup
Swine*
Vaccination
Vaccines
Vaccines

Figure

  • Fig. 1 Comparison of amino acid sequences of partial VP1 of three different serotypes between commercial vaccine strains and GenBank-deposited strains: O serotype (A), A serotype (B), and Asia 1 serotype (C). Gray shad in black box and black box indicated non-synonymous and synonymous mutations, respectively.

  • Fig. 2 Serological responses (structural protein enzyme-linked immunosorbent assay) of commercial inactivated foot-and-mouth disease virus (FMDV) vaccines: mean positive inhibition (PI) value±standard deiviation (A) and positive rates (B). The mean PI value and positive rates gradually increased until 6 weeks.


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