Korean J Parasitol.
2018 Jun;56(3):237-245. 10.3347/kjp.2018.56.3.237.
Immune Responses Induced by HSP60 DNA Vaccine against Toxoplasma gondii Infection in Kunming Mice
- Affiliations
-
- 1College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui Province 230036, P. R. China.
- 2State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, P. R. China. xingquanzhu1@hotmail.com
- 3Guangdong Wens Dahuanong Biotechnology Co., Ltd, Yunfu, Guangdong Province 524700, P. R. China.
- 4Ningbo University School of Medicine, Ningbo, Zhejiang Province 315211, P. R. China.
- 5Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu Province 225009, P. R. China.
- KMID: 2416354
- DOI: http://doi.org/10.3347/kjp.2018.56.3.237
Abstract
- Toxoplasma gondii can infect all the vertebrates including human, and leads to serious toxoplasmosis and considerable veterinary problems. T. gondii heat shock protein 60 (HSP60) is associated with the activation of antigen presenting cells by inducing initial immune responses and releasing inflammatory cytokines. It might be a potential DNA vaccine candidate for this parasite. A pVAX-HSP60 DNA vaccine was constructed and immune responses was evaluated in Kunming mice in this study. Our data indicated that the innate and adaptive immune responses was elicited by successive immunizations with pVAX-HSP60 DNA, showing apparent increases of CD3e+CD4+ and CD3e+CD8a+ T cells in spleen tissues of the HSP60 DNA-immunized mice (24.70±1.23% and 10.90±0.89%, P < 0.05) and higher levels of specific antibodies in sera. Furthermore, the survival period of the immunized mice (10.53±4.78 day) were significantly prolonged during the acute T. gondii infection. Decrease of brain cysts was significant in the experimental group during the chronic infection (P < 0.01). Taken together, TgHSP60 DNA can be as a vaccine candidate to prevent the acute and chronic T. gondii infections.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- DNA Vaccines Encoding Toxoplasma gondii Cathepsin C 1 Induce Protection against Toxoplasmosis in Mice
- Recent progress in microneme-based vaccines development against Toxoplasma gondii
- Changes in blastogenic responses and antibody titers of mice infected with Toxoplasma gondii
- Evaluation of Protective Immune Response Induced by a DNA Vaccine Encoding GRA8 against Acute Toxoplasmosis in a Murine Model
- Detection of Toxoplasma antigens and antibodies in mice infected with different strains of Toxoplasma gondii
