Nat Prod Sci.  2018 Jun;24(2):93-98. 10.20307/nps.2018.24.2.93.

Cytotoxic Triterpenoids from the Fruits of Ligustrum japonicum

• Ngo QM
• Cao TQ
• Woo MH
• Min BS
• Weon KY

• KMID: 2416068
• DOI: http://doi.org/10.20307/nps.2018.24.2.93

Abstract

Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 - 13) were isolated and evaluated for their cytotoxic activity against Hela and HL-60 cells. As results, 8 (oleanolic acid) showed significant effects on Hela with IC50 values of 5.5 µM, and moderate effects on HL-60 cells with ICâ‚…â‚€ values of 55.9 µM. Meanwhile, 10 (oleanderic acid) and 11 (3β-acetoxy-urs-12-en-28-oic acid) exhibited moderate inhibitory effects on Hela with ICâ‚…â‚€ value of 55.0 and 68.8 µM, respectively. Moreover, 10 showed cytotoxic effect on HL-60 cell line with ICâ‚…â‚€ value of 63.9 µM. To our knowledge, this is the first report that oleanderic acid was isolated from L. japonicum and investigated in cytotoxic effects on Hela and HL-60 cells.

Keyword

Ligustrum japonicum; Oleaceae; Triterpenoids; Cytotoxic activity

MeSH Terms

Antineoplastic Agents
Fruit*
HL-60 Cells
Humans
Inhibitory Concentration 50
Ligustrum*
Mass Screening
Nerium
Oleaceae
Phytochemicals
Plants, Medicinal
Antineoplastic Agents

Figure

• 

  Fig. 1. Chemical structures of triterpenoids isolated from L. japonicum.

Reference

References

(1). Jensen S. R.., Franzyk H.., Wallander E.Phytochemistry. 2002. 60:213–231.

(2). Ngo Q. -M.T.., Lee H. S.., Nguyen V. T.., Kim J. A.., Woo M. H.., Min B. S.Phytochemistry. 2017. 141:147–155.

(3). Chudzik M.., Korzonek-Szlacheta I.., Król W.Molecules. 2015. 20:1610–1625.

(4). Taher M.., Aminuddin A.., Susanti D.., Aminudin N. I.., On S.., Ahmad F.., Hamidon H.Nat. Prod. Sci. 2016. 22:122–128.

(5). Tanaka R.., Matsuda M.., Matsunaga S.Phytochemistry. 1987. 26(3365–3366):. 3365–3366.3365–3366.

(6). Pakhathirathien C.., Karalai C.., Ponglimanont C.., Subhadhirasakul S.., Chantrapromma K. J.Nat. Prod. 2005. 68:1787–1789.

(7). Chakrabartty T.., Poddar G.., Pyrek J. S.Phytochemistry. 1982. 21:1814–1816.

(8). Fuchino H.., Satoh T.., Tanaka N.Chem. Pharm. Bull. 1996. 44:1748–1753. . 1748–1753.

(9). Nomura M.., Tokoroyama T.., Kubota T.Phytochemistry. 1981. 20:1097–1104. . 1097–1104.

(10). Seebacher W.., Simic N.., Weis R.., Saf R.., Kunert O.Magn. Reson. Chem. 2003. 41:636–638.

(11). Al Musayeib N. M.., Mothana R. A.., Gamal A. A. E.., Al-Massarani S. M.., Maes L.Molecules. 2013. 18:9207–9218.

(12). Fu L.., Zhang S.., Li N.., Wang J.., Zhao M.., Sakai J.., Hasegawa T.., Mitsui T.., Kataoka T.., Oka S.., Kiuchi M.., Hirose K.., Ando M. J.Nat. Prod. 2005. 68:198–206.

(13). Batra A.., Sastry V. G.Pteridines. 2013. 24:191–199.

(14). Hota R. K.., Bapuji M.Phytochemistry. 1994. 35:1073–1074.

(15). Liao C. R.., Kuo Y. H.., Ho Y. L.., Wang C. Y.., Yang C. S.., Lin C. W.., Chang Y. S.Molecules. 2014. 19:9515–9534.

(16). Butruille D.., Dominguez X. A.Tetrahedron Lett. 1974. 15:639–642.

(17). Rouf A. S. S.., Ozaki Y.., Rashid M. A.., Rui J.Phytochemistry. 2001. 56:815–818.

(18). Zhu Y. Y.., Huang H. Y.., Wu Y. L.Mol. Med. Rep. 2015. 12:5012–5018.

(19). Akihisa T.., Kamo S.., Uchiyama T.., Akazawa H.., Banno N.., Taguchi Y.., Yasukawa K. J.Nat. Med. 2006. 60:331–333.

(20). Chiang Y. M.., Chang J. Y.., Kuo C. C.., Chang C. Y.., Kuo Y. H.Phytochemistry. 2005. 66:495–501.